Anti-Phospho-MAPK8IP1-Thr103 antibody (40-120)

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:	1 mg/mL
Dilution Range:	WB 1:500-1:2000 IHC 1:100-1:300 IF 1:200-1:1000 ELISA 1:5000
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	MAPK8IP1
Gene ID:	9479
Uniprot ID:	JIP1_HUMAN
Immunogen Region:	69-118 aa
Specificity:	Phospho-JIP-1 (T103) Polyclonal Antibody detects endogenous levels of JIP-1 protein only when phosphorylated at T103.
Immunogen:	The antiserum was produced against synthesized peptide derived from the human JIP1 around the phosphorylation site of Thr103 at the amino acid range 69-118

Post Translational Modifications	Phosphorylated by MAPK8, MAPK9 and MAPK10. Phosphorylation on Thr-103 is also necessary for the dissociation and activation of MAP3K12. Phosphorylated by isoform 1 and isoform 2 of VRK2. Hyperphosphorylated during mitosis following activation of stress-activated and MAP kinases. Ubiquitinated. Two preliminary events are required to prime for ubiquitination.phosphorylation and an increased in intracellular calcium concentration. Then, the calcium influx initiates ubiquitination and degradation by the ubiquitin-proteasome pathway.
Function	The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. Required for JNK activation in response to excitotoxic stress. Cytoplasmic MAPK8IP1 causes inhibition of JNK-regulated activity by retaining JNK in the cytoplasm and inhibiting JNK phosphorylation of c-Jun. May also participate in ApoER2-specific reelin signaling. Directly, or indirectly, regulates GLUT2 gene expression and beta-cell function. Appears to have a role in cell signaling in mature and developing nerve terminals. May function as a regulator of vesicle transport, through interactions with the JNK-signaling components and motor proteins. Functions as an anti-apoptotic protein and whose level seems to influence the beta-cell death or survival response. Acts as a scaffold protein that coordinates with SH3RF1 in organizing different components of the JNK pathway, including RAC1 or RAC2, MAP3K11/MLK3 or MAP3K7/TAK1, MAP2K7/MKK7, MAPK8/JNK1 and/or MAPK9/JNK2 into a functional multiprotein complex to ensure the effective activation of the JNK signaling pathway. Regulates the activation of MAPK8/JNK1 and differentiation of CD8(+) T-cells.
Protein Name	C-Jun-Amino-Terminal Kinase-Interacting Protein 1 Jip-1 Jnk-Interacting Protein 1 Islet-Brain 1 Ib-1 Jnk Map Kinase Scaffold Protein 1 Mitogen-Activated Protein Kinase 8-Interacting Protein 1
Cellular Localisation	Cytoplasm Perinuclear Region Nucleus Endoplasmic Reticulum Membrane Mitochondrion Membrane Accumulates In Cell Surface Projections Under Certain Stress Conditions Translocates To The Perinuclear Region Of Neurons In Insulin-Secreting Cells Detected In Both The Cytoplasm And Nucleus
Alternative Antibody Names	Anti-C-Jun-Amino-Terminal Kinase-Interacting Protein 1 antibody Anti-Jip-1 antibody Anti-Jnk-Interacting Protein 1 antibody Anti-Islet-Brain 1 antibody Anti-Ib-1 antibody Anti-Jnk Map Kinase Scaffold Protein 1 antibody Anti-Mitogen-Activated Protein Kinase 8-Interacting Protein 1 antibody Anti-MAPK8IP1 antibody Anti-IB1 antibody Anti-JIP1 antibody Anti-PRKM8IP antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance