Anti-Phospho-JAK2-Tyr221 antibody (160-240)

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:	1 mg/mL
Dilution Range:	WB 1:500-1:2000 IHC 1:100-1:300 ELISA 1:10000 IF 1:50-200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	JAK2
Gene ID:	3717
Uniprot ID:	JAK2_HUMAN
Immunogen Region:	191-240 aa
Specificity:	Phospho-JAK2 (Y221) Polyclonal Antibody detects endogenous levels of JAK2 protein only when phosphorylated at Y221.
Immunogen:	The antiserum was produced against synthesized peptide derived from the human JAK2 around the phosphorylation site of Tyr221 at the amino acid range 191-240

Post Translational Modifications	Autophosphorylated, leading to regulate its activity. Leptin promotes phosphorylation on tyrosine residues, including phosphorylation on Tyr-813. Autophosphorylation on Tyr-119 in response to EPO down-regulates its kinase activity. Autophosphorylation on Tyr-868, Tyr-966 and Tyr-972 in response to growth hormone (GH) are required for maximal kinase activity. Also phosphorylated by TEC. Phosphorylated on tyrosine residues in response to interferon gamma signaling. Phosphorylated on tyrosine residues in response to a signaling cascade that is activated by increased cellular retinol. Undergoes Notch-induced ubiquitination and subsequent proteasomal degradation which is mediated by ASB1 or ASB2, the substrate-recognition components of probable ECS E3 ubiquitin-protein ligase complexes.
Function	Non-receptor tyrosine kinase involved in various processes such as cell growth, development, differentiation or histone modifications. Mediates essential signaling events in both innate and adaptive immunity. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors such as growth hormone (GHR), prolactin (PRLR), leptin (LEPR), erythropoietin (EPOR), thrombopoietin (THPO).or type II receptors including IFN-alpha, IFN-beta, IFN-gamma and multiple interleukins. Following ligand-binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, cell stimulation with erythropoietin (EPO) during erythropoiesis leads to JAK2 autophosphorylation, activation, and its association with erythropoietin receptor (EPOR) that becomes phosphorylated in its cytoplasmic domain. Then, STAT5 is recruited, phosphorylated and activated by JAK2. Once activated, dimerized STAT5 translocates into the nucleus and promotes the transcription of several essential genes involved in the modulation of erythropoiesis. Part of a signaling cascade that is activated by increased cellular retinol and that leads to the activation of STAT5 . In addition, JAK2 mediates angiotensin-2-induced ARHGEF1 phosphorylation. Plays a role in cell cycle by phosphorylating CDKN1B. Cooperates with TEC through reciprocal phosphorylation to mediate cytokine-driven activation of FOS transcription. In the nucleus, plays a key role in chromatin by specifically mediating phosphorylation of 'Tyr-41' of histone H3 (H3Y41ph), a specific tag that promotes exclusion of CBX5 (HP1 alpha) from chromatin.
Protein Name	Tyrosine-Protein Kinase Jak2 Janus Kinase 2 Jak-2
Database Links	Reactome: R-HSA-1059683 Reactome: R-HSA-110056 Reactome: R-HSA-112411 Reactome: R-HSA-1170546 Reactome: R-HSA-1433557 Reactome: R-HSA-2586552 Reactome: R-HSA-3214858 Reactome: R-HSA-512988 Reactome: R-HSA-5673000 Reactome: R-HSA-5673001 Reactome: R-HSA-6785807 Reactome: R-HSA-6788467 Reactome: R-HSA-6802946 Reactome: R-HSA-6802952 Reactome: R-HSA-6802955 Reactome: R-HSA-69231 Reactome: R-HSA-877300 Reactome: R-HSA-877312 Reactome: R-HSA-8854691 Reactome: R-HSA-8984722 Reactome: R-HSA-9006335 Reactome: R-HSA-9020591 Reactome: R-HSA-9020933 Reactome: R-HSA-9020956 Reactome: R-HSA-9027276 Reactome: R-HSA-9027277 Reactome: R-HSA-9027283 Reactome: R-HSA-9027284 Reactome: R-HSA-912526 Reactome: R-HSA-9649948 Reactome: R-HSA-9656223 Reactome: R-HSA-9670439 Reactome: R-HSA-9674555 Reactome: R-HSA-9679191 Reactome: R-HSA-9705462 Reactome: R-HSA-9732724 Reactome: R-HSA-982772 Reactome: R-HSA-983231
Cellular Localisation	Endomembrane System Peripheral Membrane Protein Cytoplasm Nucleus
Alternative Antibody Names	Anti-Tyrosine-Protein Kinase Jak2 antibody Anti-Janus Kinase 2 antibody Anti-Jak-2 antibody Anti-JAK2 antibody

Information sourced from Uniprot.org

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