Anti-Phospho-Flk-1/Flt-4-Tyr1054/Y1063 antibody (980-1060)

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:	1 mg/mL
Dilution Range:	IHC 1:100-1:300 ELISA 1:40000 IF 1:50-200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	KDR FLT4
Gene ID:	3791 2324
Uniprot ID:	VGFR2_HUMAN VGFR3_HUMAN
Immunogen Region:	1020-1069 aa
Specificity:	Phospho-Flk-1/Flt-4 (Y1054/Y1063) Polyclonal Antibody detects endogenous levels of Flk-1/Flt-4 protein only when phosphorylated at Y1054/Y1063.
Immunogen:	The antiserum was produced against synthesized peptide derived from the human VEGFR2 around the phosphorylation site of Tyr1054 at the amino acid range 1020-1069

Post Translational Modifications	N-glycosylated. Ubiquitinated. Tyrosine phosphorylation of the receptor promotes its poly-ubiquitination, leading to its degradation via the proteasome or lysosomal proteases. Autophosphorylated on tyrosine residues upon ligand binding. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Phosphorylation at Tyr-951 is important for interaction with SH2D2A/TSAD and VEGFA-mediated reorganization of the actin cytoskeleton. Phosphorylation at Tyr-1175 is important for interaction with PLCG1 and SHB. Phosphorylation at Tyr-1214 is important for interaction with NCK1 and FYN. Dephosphorylated by PTPRB. Dephosphorylated by PTPRJ at Tyr-951, Tyr-996, Tyr-1054, Tyr-1059, Tyr-1175 and Tyr-1214. The inhibitory disulfide bond between Cys-1024 and Cys-1045 may serve as a specific molecular switch for H(2)S-induced modification that regulates KDR/VEGFR2 function.
Function	Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFC and VEGFD. Plays an essential role in the regulation of angiogenesis, vascular development, vascular permeability, and embryonic hematopoiesis. Promotes proliferation, survival, migration and differentiation of endothelial cells. Promotes reorganization of the actin cytoskeleton. Isoforms lacking a transmembrane domain, such as isoform 2 and isoform 3, may function as decoy receptors for VEGFA, VEGFC and/or VEGFD. Isoform 2 plays an important role as negative regulator of VEGFA- and VEGFC-mediated lymphangiogenesis by limiting the amount of free VEGFA and/or VEGFC and preventing their binding to FLT4. Modulates FLT1 and FLT4 signaling by forming heterodimers. Binding of vascular growth factors to isoform 1 leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, reorganization of the actin cytoskeleton and activation of PTK2/FAK1. Required for VEGFA-mediated induction of NOS2 and NOS3, leading to the production of the signaling molecule nitric oxide (NO) by endothelial cells. Phosphorylates PLCG1. Promotes phosphorylation of FYN, NCK1, NOS3, PIK3R1, PTK2/FAK1 and SRC.
Protein Name	Vascular Endothelial Growth Factor Receptor 2 Vegfr-2 Fetal Liver Kinase 1 Flk-1 Kinase Insert Domain Receptor Kdr Protein-Tyrosine Kinase Receptor Flk-1 Cd Antigen Cd309
Database Links	Reactome: R-HSA-194306 Reactome: R-HSA-195399 Reactome: R-HSA-216083 Reactome: R-HSA-4420097 Reactome: R-HSA-5218921 Reactome: R-HSA-9673768
Cellular Localisation	Cell Junction Endoplasmic Reticulum Cell Membrane Localized With Rap1a At Cell-Cell Junctions Colocalizes With Ern1 And Xbp1 In The Endoplasmic Reticulum In Endothelial Cells In A Vascular Endothelial Growth Factor (Vegf)-Dependent Manner Isoform 1: Cell Membrane Single-Pass Type I Membrane Protein Cytoplasm Nucleus Cytoplasmic Vesicle Early Endosome Detected On Caveolae-Enriched Lipid Rafts At The Cell Surface Is Recycled From The Plasma Membrane To Endosomes And Back Again Phosphorylation Triggered By Vegfa Binding Promotes Internalization And Subsequent Degradation Vegfa Binding Triggers Internalization And Translocation To The Nucleus Isoform 2: Secreted Isoform 3: Secreted
Alternative Antibody Names	Anti-Vascular Endothelial Growth Factor Receptor 2 antibody Anti-Vegfr-2 antibody Anti-Fetal Liver Kinase 1 antibody Anti-Flk-1 antibody Anti-Kinase Insert Domain Receptor antibody Anti-Kdr antibody Anti-Protein-Tyrosine Kinase Receptor Flk-1 antibody Anti-Cd Antigen Cd309 antibody Anti-KDR antibody Anti-FLK1 antibody Anti-VEGFR2 antibody

Information sourced from Uniprot.org

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