General Info
| Host: | Rabbit |
| Applications: | IHC/IF/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Rabbit polyclonal antibody anti-Phospho-Aquaporin-2-Ser256 (222-271 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | IHC 1:100-1:300ELISA 1:40000IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Information
| Gene Symbol: | AQP2 |
| Gene ID: | 359 |
| Uniprot ID: | AQP2_HUMAN |
| Immunogen Region: | 222-271 aa |
| Specificity: | Phospho-AQP2 (S256) Polyclonal Antibody detects endogenous levels of AQP2 protein only when phosphorylated at S256. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human Aquaporin 2 around the phosphorylation site of Ser256 at the amino acid range 222-271 |
Description
| Post Translational Modifications | Ser-256 phosphorylation is necessary and sufficient for expression at the apical membrane. Endocytosis is not phosphorylation-dependent. N-glycosylated. |
| Function | Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Plays an essential role in renal water homeostasis. |
| Protein Name | Aquaporin-2Aqp-2Adh Water ChannelAquaporin-CdAqp-CdCollecting Duct Water Channel ProteinWch-CdWater Channel Protein For Renal Collecting Duct |
| Database Links | Reactome: R-HSA-432040Reactome: R-HSA-432047 |
| Cellular Localisation | Apical Cell MembraneMulti-Pass Membrane ProteinBasolateral Cell MembraneCell MembraneCytoplasmic Vesicle MembraneGolgi ApparatusTrans-Golgi Network MembraneShuttles From Vesicles To The Apical MembraneVasopressin-Regulated Phosphorylation Is Required For Translocation To The Apical Cell MembranePlekha8/Fapp2 Is Required To Transport Aqp2 From The Tgn To Sites Where Aqp2 Is Phosphorylated |
| Alternative Antibody Names | Anti-Aquaporin-2 antibodyAnti-Aqp-2 antibodyAnti-Adh Water Channel antibodyAnti-Aquaporin-Cd antibodyAnti-Aqp-Cd antibodyAnti-Collecting Duct Water Channel Protein antibodyAnti-Wch-Cd antibodyAnti-Water Channel Protein For Renal Collecting Duct antibodyAnti-AQP2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance