Anti-CCN3 antibody (221-270 Internal)

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:	1 mg/mL
Dilution Range:	WB 1:500-1:2000 IHC-P 1:100-1:300 ELISA 1:20000 IF 1:50-200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	CCN3
Gene ID:	4856
Uniprot ID:	CCN3_HUMAN
Immunogen Region:	221-270 aa
Specificity:	Nov Polyclonal Antibody detects endogenous levels of Nov protein.
Immunogen:	The antiserum was produced against synthesized peptide derived from the Internal region of human NOV at the amino acid range 221-270

Post Translational Modifications	May be palmitoylated on Cys-244, which is important for extracellular secretion.
Function	Immediate-early protein playing a role in various cellular processes including proliferation, adhesion, migration, differentiation and survival. Acts by binding to integrins or membrane receptors such as NOTCH1. Essential regulator of hematopoietic stem and progenitor cell function. Inhibits myogenic differentiation through the activation of Notch-signaling pathway. Inhibits vascular smooth muscle cells proliferation by increasing expression of cell-cycle regulators such as CDKN2B or CDKN1A independently of TGFB1 signaling. Ligand of integrins ITGAV:ITGB3 and ITGA5:ITGB1, acts directly upon endothelial cells to stimulate pro-angiogenic activities and induces angiogenesis. In endothelial cells, supports cell adhesion, induces directed cell migration (chemotaxis) and promotes cell survival. Also plays a role in cutaneous wound healing acting as integrin receptor ligand. Supports skin fibroblast adhesion through ITGA5:ITGB1 and ITGA6:ITGB1 and induces fibroblast chemotaxis through ITGAV:ITGB5. Seems to enhance bFGF-induced DNA synthesis in fibroblasts. Involved in bone regeneration as a negative regulator. Enhances the articular chondrocytic phenotype, whereas it repressed the one representing endochondral ossification. Impairs pancreatic beta-cell function, inhibits beta-cell proliferation and insulin secretion. Plays a role as negative regulator of endothelial pro-inflammatory activation reducing monocyte adhesion, its anti-inflammatory effects occur secondary to the inhibition of NF-kappaB signaling pathway. Contributes to the control and coordination of inflammatory processes in atherosclerosis. Attenuates inflammatory pain through regulation of IL1B- and TNF-induced MMP9, MMP2 and CCL2 expression. Inhibits MMP9 expression through ITGB1 engagement.
Protein Name	Ccn Family Member 3 Cellular Communication Network Factor 3 Insulin-Like Growth Factor-Binding Protein 9 Ibp-9 Igf-Binding Protein 9 Igfbp-9 Nephro Blastoma-Overexpressed Gene Protein Homolog Protein Nov Homolog Novh
Cellular Localisation	Secreted Cytoplasm Cell Junction Gap Junction Localizes At The Gap Junction In Presence Of Gja1
Alternative Antibody Names	Anti-Ccn Family Member 3 antibody Anti-Cellular Communication Network Factor 3 antibody Anti-Insulin-Like Growth Factor-Binding Protein 9 antibody Anti-Ibp-9 antibody Anti-Igf-Binding Protein 9 antibody Anti-Igfbp-9 antibody Anti-Nephro Blastoma-Overexpressed Gene Protein Homolog antibody Anti-Protein Nov Homolog antibody Anti-Novh antibody Anti-CCN3 antibody Anti-IGFBP9 antibody Anti-NOV antibody Anti-NOVH antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance