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Reagents and instruments for immunology, cell biology and molecular biology.

Anti-NOS3 antibody (1120-1200)

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Cat# NB-22-4537-200ul

Size : 200µg

Contact local distributor :

Phone : +1 850 650 7790

Brand : Neo Biotech

Contact local distributor :

Phone : +1 850 650 7790

Western blot analysis of mouse-brain lysis using NOS3 antibody. Antibody was diluted at 1:1000
Western blot analysis of A549 cells using NOS3 Polyclonal Antibody diluted at 1：1000
Western blot analysis of A549 cells using NOS3 Polyclonal Antibody diluted at 1：1000
Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue. 1, NOS3 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1, NOS3 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Western blot analysis of lysates from A549, K562, and COLO205 cells, using eNOS Antibody. The lane on the right is blocked with the synthesized peptide.
Immunofluorescence analysis of rat-lung tissue. 1, NOS3 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1, NOS3 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-stomach tissue. 1, NOS3 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunofluorescence analysis of rat-spleen tissue. 1, NOS3 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of rat-spleen tissue. 1, NOS3 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of rat-kidney tissue. 1, NOS3 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of rat-kidney tissue. 1, NOS3 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of rat-lung tissue. 1, NOS3 Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Western blot analysis of mouse-brain lysis using NOS3 antibody. Antibody was diluted at 1:1000
Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1, NOS3 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunofluorescence analysis of HeLa cells, using eNOS Antibody. The picture on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded Rat-brain tissue. 1, NOS3 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Western blot analysis of the lysates from HepG2 cells using eNOS antibody.
Immunofluorescence analysis of Hela cell. 1, NOS3 Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

General Info

Host:	Rabbit
Applications:	WB/IHC/IF/ELISA
Reactivity:	Human/Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit polyclonal antibody anti-Nitric oxide synthase 3 (1145-1194) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:	1 mg/mL
Dilution Range:	WB 1:500-2000 IF 1:50-300 IHC 1:50-300
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Information

Gene Symbol:	NOS3
Gene ID:	4846
Uniprot ID:	NOS3_HUMAN
Immunogen Region:	1145-1194
Specificity:	NOS3 Polyclonal Antibody detects endogenous levels of NOS3 protein.
Immunogen:	The antiserum was produced against synthesized peptide derived from human eNOS. AA range:1145-1194

Description

Tissue Specificity	Platelets, placenta, liver and kidney.
Post Translational Modifications	Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity. Phosphorylation of Ser-114 by CDK5 reduces activity.
Function	Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.
Protein Name	Nitric Oxide Synthase 3 Constitutive Nos Cnos Ec-Nos Nos Type Iii Nosiii Nitric Oxide Synthase - Endothelial Endothelial Nos Enos
Database Links	Reactome: R-HSA-1222556 Reactome: R-HSA-1474151 Reactome: R-HSA-203615 Reactome: R-HSA-203641 Reactome: R-HSA-203754 Reactome: R-HSA-392154 Reactome: R-HSA-5218920 Reactome: R-HSA-9009391
Cellular Localisation	Cell Membrane Membrane Caveola Cytoplasm Cytoskeleton Golgi Apparatus Specifically Associates With Actin Cytoskeleton In The G2 Phase Of The Cell Cycle Which Is Favored By Interaction With Nosip And Results In A Reduced Enzymatic Activity
Alternative Antibody Names	Anti-Nitric Oxide Synthase 3 antibody Anti-Constitutive Nos antibody Anti-Cnos antibody Anti-Ec-Nos antibody Anti-Nos Type Iii antibody Anti-Nosiii antibody Anti-Nitric Oxide Synthase - Endothelial antibody Anti-Endothelial Nos antibody Anti-Enos antibody Anti-NOS3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance