Anti-GRK5 antibody (320-400 Internal)

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:	1 mg/mL
Dilution Range:	WB 1:500-1:2000 IHC 1:100-1:300 ELISA 1:10000 IF 1:50-200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	GRK5
Gene ID:	2869
Uniprot ID:	GRK5_HUMAN
Immunogen Region:	351-400 aa
Specificity:	GRK 5 Polyclonal Antibody detects endogenous levels of GRK 5 protein.
Immunogen:	The antiserum was produced against synthesized peptide derived from the human GRK5 at the amino acid range 351-400

Post Translational Modifications	Autophosphorylated. Autophosphorylation may play a critical role in the regulation of GRK5 kinase activity.
Function	Serine/threonine kinase that phosphorylates preferentially the activated forms of a variety of G-protein-coupled receptors (GPCRs). Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their down-regulation. Phosphorylates a variety of GPCRs, including adrenergic receptors, muscarinic acetylcholine receptors (more specifically Gi-coupled M2/M4 subtypes), dopamine receptors and opioid receptors. In addition to GPCRs, also phosphorylates various substrates: Hsc70-interacting protein/ST13, TP53/p53, HDAC5, and arrestin-1/ARRB1. Phosphorylation of ARRB1 by GRK5 inhibits G-protein independent MAPK1/MAPK3 signaling downstream of 5HT4-receptors. Phosphorylation of HDAC5, a repressor of myocyte enhancer factor 2 (MEF2) leading to nuclear export of HDAC5 and allowing MEF2-mediated transcription. Phosphorylation of TP53/p53, a crucial tumor suppressor, inhibits TP53/p53-mediated apoptosis. Phosphorylation of ST13 regulates internalization of the chemokine receptor. Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor, LRP6 during Wnt signaling (in vitro).
Protein Name	G Protein-Coupled Receptor Kinase 5 G Protein-Coupled Receptor Kinase Grk5
Database Links	Reactome: R-HSA-416476 Reactome: R-HSA-418555
Cellular Localisation	Cytoplasm Nucleus Cell Membrane Peripheral Membrane Protein Predominantly Localized At The Plasma Membrane Targeted To The Cell Surface Through The Interaction With Phospholipids Nucleus Localization Is Regulated In A Gpcr And Ca(2+)/Calmodulin-Dependent Fashion
Alternative Antibody Names	Anti-G Protein-Coupled Receptor Kinase 5 antibody Anti-G Protein-Coupled Receptor Kinase Grk5 antibody Anti-GRK5 antibody Anti-GPRK5 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance