de en

Reagents and instruments for immunology, cell biology and molecular biology.

Anti-GRK2 antibody (570-650 C-Term)

Print

Cat# NB-22-3427-200ul

Size : 200µg

Contact local distributor :

Phone : +1 850 650 7790

Brand : Neo Biotech

Contact local distributor :

Phone : +1 850 650 7790

Western blot analysis of lysates from HUVEC cells, using ARBK1 Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded Human Lymph gland. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human Lymph gland. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Western blot analysis of HuvEc cells using GRK 2 Polyclonal Antibody
Western blot analysis of various cells using GRK 2 Polyclonal Antibody

General Info

Host:	Rabbit
Applications:	WB/IHC/IF/ELISA
Reactivity:	Human/Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit polyclonal antibody anti-Beta-adrenergic receptor kinase 1 (601-650 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:	1 mg/mL
Dilution Range:	WB 1:500-1:2000 IHC 1:100-1:300 ELISA 1:20000 IF 1:50-200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Information

Gene Symbol:	GRK2
Gene ID:	156
Uniprot ID:	ARBK1_HUMAN
Immunogen Region:	601-650 aa
Specificity:	GRK 2 Polyclonal Antibody detects endogenous levels of GRK 2 protein.
Immunogen:	The antiserum was produced against synthesized peptide derived from the human ARBK1 at the amino acid range 601-650

Description

Function	Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. Key regulator of LPAR1 signaling. Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor. Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner. Positively regulates ciliary smoothened (SMO)-dependent Hedgehog (Hh) signaling pathway by facilitating the trafficking of SMO into the cilium and the stimulation of SMO activity. Inhibits relaxation of airway smooth muscle in response to blue light.
Protein Name	Beta-Adrenergic Receptor Kinase 1 Beta-Ark-1 G-Protein Coupled Receptor Kinase 2
Database Links	Reactome: R-HSA-111933 Reactome: R-HSA-416476 Reactome: R-HSA-418555 Reactome: R-HSA-5635838 Reactome: R-HSA-8856825
Cellular Localisation	Cytoplasm Cell Membrane Postsynapse Presynapse
Alternative Antibody Names	Anti-Beta-Adrenergic Receptor Kinase 1 antibody Anti-Beta-Ark-1 antibody Anti-G-Protein Coupled Receptor Kinase 2 antibody Anti-GRK2 antibody Anti-ADRBK1 antibody Anti-BARK antibody Anti-BARK1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance