Anti-DDIT3 antibody (10-90)

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:	1 mg/mL
Dilution Range:	WB 1:500-1:2000 IHC 1:100-1:300 IF 1:200-1:1000 ELISA 1:5000
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	DDIT3
Gene ID:	1649
Uniprot ID:	DDIT3_HUMAN
Immunogen Region:	15-64 aa
Specificity:	CHOP Polyclonal Antibody detects endogenous levels of CHOP protein.
Immunogen:	The antiserum was produced against synthesized peptide derived from the human CHOP at the amino acid range 15-64

Function	Multifunctional transcription factor in endoplasmic reticulum (ER) stress response. Plays an essential role in the response to a wide variety of cell stresses and induces cell cycle arrest and apoptosis in response to ER stress. Plays a dual role both as an inhibitor of CCAAT/enhancer-binding protein (C/EBP) function and as an activator of other genes. Acts as a dominant-negative regulator of C/EBP-induced transcription: dimerizes with members of the C/EBP family, impairs their association with C/EBP binding sites in the promoter regions, and inhibits the expression of C/EBP regulated genes. Positively regulates the transcription of TRIB3, IL6, IL8, IL23, TNFRSF10B/DR5, PPP1R15A/GADD34, BBC3/PUMA, BCL2L11/BIM and ERO1L. Negatively regulates.expression of BCL2 and MYOD1, ATF4-dependent transcriptional activation of asparagine synthetase (ASNS), CEBPA-dependent transcriptional activation of hepcidin (HAMP) and CEBPB-mediated expression of peroxisome proliferator-activated receptor gamma (PPARG). Together with ATF4, mediates ER-mediated cell death by promoting expression of genes involved in cellular amino acid metabolic processes, mRNA translation and the unfolded protein response (UPR) in response to ER stress. Inhibits the canonical Wnt signaling pathway by binding to TCF7L2/TCF4, impairing its DNA-binding properties and repressing its transcriptional activity. Plays a regulatory role in the inflammatory response through the induction of caspase-11 (CASP4/CASP11) which induces the activation of caspase-1 (CASP1) and both these caspases increase the activation of pro-IL1B to mature IL1B which is involved in the inflammatory response. Acts as a major regulator of postnatal neovascularization through regulation of endothelial nitric oxide synthase (NOS3)-related signaling.
Protein Name	Dna Damage-Inducible Transcript 3 Protein Ddit-3 C/Ebp Zeta C/Ebp-Homologous Protein Chop C/Ebp-Homologous Protein 10 Chop-10 Ccaat/Enhancer-Binding Protein Homologous Protein Growth Arrest And Dna Damage-Inducible Protein Gadd153
Database Links	Reactome: R-HSA-380994 Reactome: R-HSA-381183 Reactome: R-HSA-9614657 Reactome: R-HSA-9633012 Reactome: R-HSA-9648895
Cellular Localisation	Cytoplasm Nucleus Present In The Cytoplasm Under Non-Stressed Conditions And Er Stress Leads To Its Nuclear Accumulation
Alternative Antibody Names	Anti-Dna Damage-Inducible Transcript 3 Protein antibody Anti-Ddit-3 antibody Anti-C/Ebp Zeta antibody Anti-C/Ebp-Homologous Protein antibody Anti-Chop antibody Anti-C/Ebp-Homologous Protein 10 antibody Anti-Chop-10 antibody Anti-Ccaat/Enhancer-Binding Protein Homologous Protein antibody Anti-Growth Arrest And Dna Damage-Inducible Protein Gadd153 antibody Anti-DDIT3 antibody Anti-CHOP antibody Anti-CHOP10 antibody Anti-GADD153 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance