TNF ELISA Kit (Pig)
Cat# OKEH04623
Size : 96Wells
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
| Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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| Predicted Species Reactivity | Porcine, Sus scrofa | ||||||||||||||||||||||
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| Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology TNF ELISA Kit (Pig) (OKEH04623) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TNF has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for TNF is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TNF captured in well. | ||||||||||||||||||||||
| ELISA Kit Range | 31.2-2000pg/mL | ||||||||||||||||||||||
| ELISA Kit Recovery | <= 5.1% | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Mean Intra-Assay CV: < 10% Mean Inter-Assay CV: < 12% | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids | ||||||||||||||||||||||
| Sensitivity | 8.4 pg/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant Pig Tumor necrosis factor | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | TNF |
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| Gene Full Name | tumor necrosis factor |
| Alias Symbols | cachectin;TNFa;TNF-a;TNF-alpha;tumor necrosis factor;tumor necrosis factor (TNF superfamily, member 2);tumor necrosis factor alpha;tumor necrosis factor ligand superfamily member 2. |
| NCBI Gene Id | 397086 |
| Protein Name | P23563 |
| Description of Target | Tumor necrosis factor |
| Uniprot ID | https://www.uniprot.org/uniprot/P23563 |
| Protein Accession # | NP_999187.1 |
| Nucleotide Accession # | NM_214022.1 |
| Protein Size (# AA) | 232 |
| Molecular Weight | 25 KDa |