Free Tri-iodothyronine ELISA Kit (Pig)
Cat# OKCA00320
Size : 96Wells
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
| Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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| Predicted Species Reactivity | Pig | ||||||||||||||||||||||||||||||||||||||||||
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| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||||||||||||||||||||||
| ELISA Kit Duration | < 4 Hours | ||||||||||||||||||||||||||||||||||||||||||
| ELISA Kit Linearity |
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| ELISA Kit Principle | Aviva Systems Biology Free Tri-iodothyronine ELISA Kit (Pig) (OKCA00320) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Pig Free Tri-iodothyronine antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Free Tri-iodothyronine and incubated. The Free Tri-iodothyronine found in the sample or standards competes with the biotinylated Free Tri-iodothyronine for limited binding sites on the immobilized anti-Pig Free Tri-iodothyronine antibody. Excess unbound biotinylated Free Tri-iodothyronine and sample or standard Free Tri-iodothyronine is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Free Tri-iodothyronine captured in the well and inversely proportional to the amount of Free Tri-iodothyronine which was contained in the sample or standard. | ||||||||||||||||||||||||||||||||||||||||||
| ELISA Kit Range | 2 pmol/L - 32 pmol/L | ||||||||||||||||||||||||||||||||||||||||||
| ELISA Kit Recovery |
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| ELISA Kit Reproducibility |
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| ELISA Kit Component |
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| Additional Information | CAS#: 6893-02-3 Molecular Formula: C15H12I3NO4 Molecular Weight: 650.9776 g mol−1 | ||||||||||||||||||||||||||||||||||||||||||
| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||||||||||||||||||||||
| Sample Type | Serum, Plasma, Cell culture supernates and Tissue homogenates | ||||||||||||||||||||||||||||||||||||||||||
| Sensitivity | < 0.38 pmoL/L | ||||||||||||||||||||||||||||||||||||||||||
| Specificity | This assay has high sensitivity and excellent specificity for detection of sheep FT3. No significant cross-reactivity or interference between sheep FT3 and analogues was observed. | ||||||||||||||||||||||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Competitive Immunoassay | ||||||||||||||||||||||||||||||||||||||||||
| Gene Symbol | Free Tri-iodothyronine |
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| Alias Symbols | 3,3',5-Triiodo-L-thyronine, 3,3',5-Triiodothyronine, 3,5,3'-Triiodothyronine, 4-(4-Hydroxy-3-iodophenoxy)-3,5-diiodophenylalanine, Alanine, 3-[4-(4-hydroxy-3-iodophenoxy)-3,5-diiodophenyl]-, L-Cyronine, L-3,3',5-Triiodothyronine, othyronin, Liothyronin, l |
| Protein Name | Free Tri-iodothyronine |
| Description of Target | An iodothyronine compound having iodo substituents at the 3-, 3'- and 5-positions. Although some is produced in the thyroid, most of the 3,3',5-triiodo-L-thyronine in the body is generated by mono-deiodination of L-thyroxine in the peripheral tissues. Its metabolic activity is about 3 to 5 times that of L-thyroxine. The sodium salt is used in the treatment of hypothyroidism. |