DAO1 ELISA Kit (Pig)

Cat# OKCD02488

Size : 96Wells

Brand : Aviva Systems Biology

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Phone : +1 850 650 7790

Datasheets/ManualsPrintable datasheet for DAO1 ELISA Kit (Pig) (OKCD02488)
Product Info
Predicted Species ReactivityPorcine|Sus scrofa
ApplicationELISA-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)97-105%79-104%97-105%93-105%
EDTA Plasma (n=5)88-102%83-101%82-93%80-89%
Heparin Plasma (n=5)85-101%96-104%84-101%93-101%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Amiloride Binding Protein 1 (ABP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Amiloride Binding Protein 1 (ABP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Amiloride Binding Protein 1 (ABP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Amiloride Binding Protein 1 (ABP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range3.12-200ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)81-9785
EDTA Plasma (n=5)90-9794
Heparin Plasma(n=5)82-9893
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Amiloride Binding Protein 1 (ABP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Amiloride Binding Protein 1 (ABP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-DAO1 Microplate96 Wells (12 x 8 Well strips)
DAO1 Lyophilized Standard2
100X Biotinylated DAO1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity1.17 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Diamine Oxidase (DAO). No significant cross-reactivity or interference between Diamine Oxidase (DAO) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
  • Reconstitution & Storage Instructions
  • Western Blotting/Immunoblotting (WB/IB) Protocol
  • Immunohistochemistry (IHC) Protocol
  • Immunocytochemistry (ICC) Protocol
  • Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
  • Blocking Peptide Competition Protocol (BPCP)
  • Immunoprecipitation (IP) Protocol
  • Antibody Array (AA) Protocol
Gene SymbolAOC1
Gene Full Nameamine oxidase copper containing 1
Alias SymbolsABP1;amiloride binding protein 1 (amine oxidase (copper-containing));Amiloride-binding protein 1;Amiloride-sensitive amine oxidase;amiloride-sensitive amine oxidase [copper-containing];Amine oxidase copper domain-containing protein 1;DAO;Diamine oxidase;Histaminase.
NCBI Gene Id100517436
Protein NameAmiloride-sensitive amine oxidase [copper-containing]
Description of TargetCatalyzes the degradation of compounds such as putrescine, histamine, spermine, and spermidine, substances involved in allergic and immune responses, cell proliferation, tissue differentiation, tumor formation, and possibly apoptosis.
Uniprot IDQ9TRC7
Protein Accession #NP_999231.1
Nucleotide Accession #NM_214066.2
Protein Size (# AA)347