DAO1 ELISA Kit (Pig)
Cat# OKCD02488
Size : 96Wells
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
| Datasheets/Manuals | Printable datasheet for DAO1 ELISA Kit (Pig) (OKCD02488) |
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| Predicted Species Reactivity | Porcine|Sus scrofa | ||||||||||||||||||||||
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| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
| ELISA Kit Duration | 3h | ||||||||||||||||||||||
| ELISA Kit Linearity |
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| ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Amiloride Binding Protein 1 (ABP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Amiloride Binding Protein 1 (ABP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Amiloride Binding Protein 1 (ABP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Amiloride Binding Protein 1 (ABP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
| ELISA Kit Range | 3.12-200ng/mL | ||||||||||||||||||||||
| ELISA Kit Recovery |
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| ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Amiloride Binding Protein 1 (ABP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Amiloride Binding Protein 1 (ABP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
| Sample Type | Serum, plasma, tissue homogenates and other biological fluids | ||||||||||||||||||||||
| Sensitivity | 1.17 ng/mL | ||||||||||||||||||||||
| Specificity | This assay has high sensitivity and excellent specificity for detection of Diamine Oxidase (DAO). No significant cross-reactivity or interference between Diamine Oxidase (DAO) and analogues was observed. | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA | ||||||||||||||||||||||
| Protocol Information |
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| Gene Symbol | AOC1 |
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| Gene Full Name | amine oxidase copper containing 1 |
| Alias Symbols | ABP1;amiloride binding protein 1 (amine oxidase (copper-containing));Amiloride-binding protein 1;Amiloride-sensitive amine oxidase;amiloride-sensitive amine oxidase [copper-containing];Amine oxidase copper domain-containing protein 1;DAO;Diamine oxidase;Histaminase. |
| NCBI Gene Id | 100517436 |
| Protein Name | Amiloride-sensitive amine oxidase [copper-containing] |
| Description of Target | Catalyzes the degradation of compounds such as putrescine, histamine, spermine, and spermidine, substances involved in allergic and immune responses, cell proliferation, tissue differentiation, tumor formation, and possibly apoptosis. |
| Uniprot ID | Q9TRC7 |
| Protein Accession # | NP_999231.1 |
| Nucleotide Accession # | NM_214066.2 |
| Protein Size (# AA) | 347 |