ACTH ELISA Kit (Pig)
Cat# OKEH00626
Size : 96T
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
| Predicted Species Reactivity | Porcine, Sus scrofa | ||||||||||||||||||||||
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| Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology ACTH ELISA Kit (Pig) (OKEH00626) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ACTH has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ACTH is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ACTH captured in well. | ||||||||||||||||||||||
| ELISA Kit Range | 78-5000pg/mL | ||||||||||||||||||||||
| ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101% | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Mean Intra-assay CV%: < 6.1% (n = 20) Mean Inter-assay CV%: < 10.9% (n = 20) | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids | ||||||||||||||||||||||
| Sensitivity | < 7.5 pg/mL | ||||||||||||||||||||||
| Specificity | This assay recognizes recombinant and natural pig Corticotropin. No significant cross-reactivity or interference was observed. | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich Immunoassay |
| Gene Symbol | POMC |
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| Gene Full Name | proopiomelanocortin |
| Alias Symbols | corticotropin-lipotropin;pro-opiomelanocortin;proopiomelanocortin (adrenocorticotropin/ beta-lipotropin/ alpha-melanocyte stimulating hormone/ beta-melanocyte stimulating hormone/ beta-endorphin). |
| NCBI Gene Id | 396863 |
| Protein Name | P01192 |
| Description of Target | Pro-opiomelanocortin |
| Uniprot ID | https://www.uniprot.org/uniprot/P01192 |
| Protein Accession # | NP_999023.1 |
| Nucleotide Accession # | NM_213858.1 |
| Protein Size (# AA) | 191 |
| Molecular Weight | 21 kDa |