COL1A1 ELISA Kit (Pig)
Cat# OKEH04797
Size : 96Wells
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
| Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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| Predicted Species Reactivity | Pig | ||||||||||||||||||||||
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| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology col1a1 ELISA Kit (Pig) (OKEH04797) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for col1a1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for col1a1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample col1a1 captured in well. | ||||||||||||||||||||||
| ELISA Kit Range | 1.56 - 100 ng/mL | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Mean Intra-Assay CV: < 10% Mean Inter-Assay CV: < 12% | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids. | ||||||||||||||||||||||
| Sensitivity | < 0.39 ng/mL | ||||||||||||||||||||||
| Specificity | This assay recognizes recombinant and natural pig Collagen alpha-1(I) chain. No significant cross-reactivity or interference was observed. | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich Immunoassay |
| Gene Symbol | COL1A1 |
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| NCBI Gene Id | 100733209 |
| Protein Name | Type I collagen alpha 1 chain |
| Uniprot ID | Q9MYT7 |
| Protein Accession # | XP_013004992.1 ? |
| Nucleotide Accession # | XM_013149538.1 |
| Protein Size (# AA) | 15 |
| Molecular Weight | 2 kDa |