C3 ELISA Kit (Guinea Pig)
Cat# OKIA00034
Size : 1x96wellplate
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
| Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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| Predicted Species Reactivity | Guinea Pig | ||||||||||||||||
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| Product Format | In Solution | ||||||||||||||||
| Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||
| ELISA Kit Duration | 25 Minutes | ||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology C3 ELISA Kit (Guinea Pig) (OKIA00034) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Complement C3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Complement C3 - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Complement C3 captured in the well. | ||||||||||||||||
| ELISA Kit Range | 3.125 ng/ml - 200 ng/ml | ||||||||||||||||
| ELISA Kit Component |
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| Additional Information | Intended Use: The Complement factor 3 (C3) test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring C3 in biological samples of Guinea Pigs. | ||||||||||||||||
| :: | Reagents: 1. DILUENT CONCENTRATE (Running Buffer) One bottle containing 50 ml of a 5X concentrated diluent running buffer. 2. WASH SOLUTION CONCENTRATE One bottle containing 50 ml of a 20X concentrated wash solution. 3. ENZYME-ANTIBODY CONJUGATE 100X One vial containing 150 ul of affinity purified anti-Guinea Pig C3 antibody conjugated with horseradish peroxidase in a stabilizing buffer. 4. CHROMOGEN-SUBSTRATE SOLUTION One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3. 5. STOP SOLUTION One vial containing 12 ml 0.3 M sulfuric acid. WARNING: Avoid contact with skin. 6. ANTI-GUINEA PIG C3 ELISA MICRO PLATE Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Guinea Pig C3. 7. GUINEA PIG C3 STANDARDS One vial containing Guinea Pig C3 calibrator. | ||||||||||||||||
| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||
| Sample Type | Serum, plasma and other biological fluids | ||||||||||||||||
| Sensitivity | 1.399 ng/ml | ||||||||||||||||
| Predicted Homology Based on Immunogen Sequence | Guinea Pig | ||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Alias Symbols | Complement C3, C3, CPAMD1 |
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| NCBI Gene Id | 100135462 |
| Protein Name | Complement C3 |
| Description of Target | C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. In chronic inflammation, acts as a chemoattractant for neutrophils.C3-beta-c: Acts as a chemoattractant for neutrophils in chronic inflammation. |
| Uniprot ID | P12387 |