Villin (GI-Mucosal & Urogenital Brush Border Marker) (VIL1/1325), APC conjugate, 0.1mg/mL

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Cat# BNCA1325-250

Size : 250uL

Contact local distributor :

Phone : +1 850 650 7790

Brand : Biotium

Contact local distributor :

Phone : +1 850 650 7790

Villin Monoclonal Mouse Antibody (VIL1/1325)

Product Attributes

Antibody number	#1325
Antibody reactivity (target)	Villin
Antibody type	Primary
Host species	Mouse
Clonality	Monoclonal
Clone	VIL1/1325
Isotype	IgG1, kappa
Molecular weight	93 kDa
Synonyms	VIL1; Villin-1; Villin1
Human gene symbol	VIL1
Entrez gene ID	7429
SwissProt	P09327
Unigene	654595
Immunogen	Recombinant human Villin fragment of 133 amino acid residues (aa179-311) (exact sequence is proprietary)
Antibody target cellular localization	Cytoskeleton
Verified antibody applications	Flow (intracellular) (verified), IF (verified), IHC (FFPE) (verified), WB (verified)
Species reactivity	Human
Antibody application notes	Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody, Immunofluorescence: 1-2 ug/mL, Flow cytometry: 0.5-1 ug/million cells in 0.1mL, Immunohistology (formalin): 0.25-0.5 ug/mL, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min, Western blotting 1-2 ug/mL, Optimal dilution for a specific application should be determined by user
Positive control	A549, HepG2 and HCT116 cells. Colon or Rectum.
Shipping condition	Room temperature
Storage Conditions	Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C
Shelf life	Guaranteed for at least 24 months from date of receipt when stored as recommended
Regulatory status	For research use only (RUO)
Antibody/conjugate formulation	Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide
Antibody research areas	Cancer
Cell/tissue expression	Epithelial cells, Intestine
Tumor expression	Colorectal cancer

Product Description

This antibody recognizes a protein of 95 kDa, which is identified as villin. It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, villin is predominantly expressed in tumors of colorectal origin. Antibody to villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.

Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the CF® Dye Brochure for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Stock status: Because Biotium offers a large number of antibody and conjugation options, primary antibody conjugates may be made to order. Typical lead times are up to one week for CF® dye and biotin conjugates, and up to 2-3 weeks for fluorescent protein and enzyme conjugates. Please email info@biotrend.com to inquire about stock status and lead times before placing your order.

Catalog Number Key for Primary Antibody Conjugates

Antibody # prefix	Conjugation	Ex/Em (nm)	Laser line	Detection channel	Dye Features
BNC04	CF®405S	404/431	405	DAPI (microscopy), AF405	CF®405S Features
BNC88	CF®488A	490/515	488	GFP, FITC	CF®488A Features
BNC68	CF®568	562/583	532, 561	RFP, TRITC	CF®568 Features
BNC94	CF®594	593/614	561	Texas Red®	CF®594 Features
BNC40	CF®640R	642/662	633-640	Cy®5	CF®640R Features
BNC47	CF®647	650/665	633-640	Cy®5	CF®647 Features
BNCB	Biotin	N/A	N/A	N/A
BNUB	Purified	N/A	N/A	N/A
BNUM	Purified, BSA-free	N/A	N/A	N/A

Alexa Fluor, Pacific Blue, Pacific Orange, and Texas Red are trademarks or registered trademarks of Thermo Fisher Scientific; Cy is a registered trademark of Cytiva; IRDye, LI-COR, and Odyssey are registered trademarks of LI-COR Bioscience.