Product Description  

VAHTS Small RNA Library Prep Kit for Illumina V2 is a small RNA-seq library preparation kit specifically designed for Illumina high-throughput sequencing platforms. This kit improves the stability and library yield at a lower-input amount through system optimisation. It is intended for animal and plant total RNA with a starting template amount of 10 ng - 1 μg, and ≥1 ng of RNA extracted and purified from serum, plasma, and exosomes. The universal adaptors are ligated to 3' and 5' ends of small RNAs, respectively. After steps such as reverse transcription, PCR amplification, and PAGE gel or magnetic bead purification, the sequencing library suitable for the Illumina platform can be obtained. This kit contains all the enzymes and buffers required for library preparation. All the reagents provided in the kit have undergone rigorous quality control and functional testing to ensure the optimal stability and repeatability of library preparation.

 Features  

• Wide range of compatible samples: animal and plant total RNA, special sample (serum, plasma, exosome) RNA, FFPE total RNA, isolatedand purified small RNA.

• Compatible with low input samples: 10 ng-1 μg animal and plant total RNA, ≥1 ng serum, plasma, exosomal RNA.

•  Increased success rate of library construction for special samples: increased success rate of library construction for serum, plasma, and exosomal RNA.

• High conversion rate of effective library: 3' adapter ligation efficiency and reverse transcription efficiency are improved, which improves the output of effective library.

• Good quality of sequencing data: the expression repeat correlation is good, and the types of miRNA detection are improved.

  
  

 Components  

 Storage  

RL5 Adaptor: Store at -85 ~ -65°C and ship on dry ice. 

Filtration Column: Store at 15 ~ 25°C and transport at room temperature. 

Other components: Store at -30 ~ -15°C and transport at ≤0°C.