TRIM5 Antibody
Cat# OAPB00225
Size : 0.1mg
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
TRIM5 Antibody (OAPB00225)
| Datasheets/Manuals | Printable datasheet for TRIM5 Antibody (OAPB00225) (OAPB00225) |
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| Predicted Species Reactivity | Human|Rhesus Monkey |
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| Product Format | Liquid. TRIM5 alpha Antibody is supplied in PBS containing 0.02% sodium azide. |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugation | Unconjugated |
| Application | ELISA, IHC-P, WB |
| :: | Buffer: Antibody is supplied in PBS containing 0.02% sodium azide. |
| Reconstitution and Storage | TRIM5 alpha antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Immunogen | TRIM5 alpha antibody was raised against a synthetic peptide corresponding to 19 amino acids near the center of rhesus monkey TRIM5 alpha. The immunogen is located within amino acids 390 - 440 of TRIM5 alpha. |
| Purification | Affinity Purified |
| Predicted Homology Based on Immunogen Sequence | Human |
| Concentration | 1 mg/ml |
| Blocking Peptide | For anti-TRIM5 (OAPB00225) antibody is Catalog # OEPB02319 |
| Application Info | TRIM5 alpha antibody can be used for detection of TRIM5 alpha by Western blot at 1 to 2 ug/ml. A band at approximately 55 kDa can be detected. (Optimal dilution should be determined by user) |
| Reference | Reymond A, Meroni G, Fantozzi A, et al. The tripartite motif family identifies cell compartments. EMBO J. 2001; 20:2140-51.;Stremlau M, Owens CM, Perron MJ, et al. The cytoplasmic body component TRIM5alpha restricts HIV-1 infection in Old World monkeys. Nature 2004; 427:848-53.;Hatziioannou T, Perez-Caballero D, Yang A, et al. Retrovirus resistance factors REF1 and Lv1 are species-specific variants of TRIM5alpha. Proc. Nat’l. Acad. Sci. USA 2004; 101:10774-9; |
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| Gene Symbol | TRIM5 |
| Gene Full Name | tripartite motif containing 5 |
| Alias Symbols | RING-type E3 ubiquitin transferase TRIM5;TRIM5alpha;tripartite motif-containing 5/cyclophilin A fusion protein;tripartite motif-containing protein 5. |
| NCBI Gene Id | 574288 |
| Protein Name | tripartite motif-containing 5 alpha isoform [Macaca mulatta]|Tripartite motif-containing protein 5 |
| Description of Target | Capsid-specific restriction factor that prevents infection from non-host-adapted retroviruses. Blocks viral replication early in the life cycle, after viral entry but before reverse transcription. In addition to acting as a capsid-specific restriction factor, also acts as a pattern recognition receptor that activates innate immune signaling in response to the retroviral capsid lattice. Binding to the viral capsid triggers its E3 ubiquitin ligase activity, and in concert with the heterodimeric ubiquitin conjugating enzyme complex UBE2V1-UBE2N (also known as UBC13-UEV1A complex) generates 'Lys-63'-linked polyubiquitin chains, which in turn are catalysts in the autophosphorylation of the MAP3K7/TAK1 complex (includes TAK1, TAB2, and TAB3). Activation of the MAP3K7/TAK1 complex by autophosphorylation results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes, thereby leading to an innate immune response in the infected cell. Restricts infection by human immunodeficiency virus type 1 (HIV-1) and simian immunodeficiency virus (SIV-agm). Plays a role in regulating autophagy through activation of autophagy regulator BECN1 by causing its dissociation from its inhibitors BCL2 and TAB2 (PubMed:25127057). Also plays a role in autophagy by acting as a selective autophagy receptor which recognizes and targets HIV-1 capsid protein p24 for autophagic destruction (PubMed:25127057). |
| Uniprot ID | Q0PF16 |
| Protein Accession # | https://www.ncbi.nlm.nih.gov/protein/ABG67967 |
| Nucleotide Accession # | NM_033034.2; NM_033092.2; NM_033093.2; XM_005253183.1; XM_005253184.1 |
| Molecular Weight | Predicted: 54 kDa Observed: 54 kDa |
