TPH2 ELISA Kit (Mouse)
Cat# OKCD02950
Size : 96Wells
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
| Datasheets/Manuals | Printable datasheet for TPH2 ELISA Kit (Mouse) (OKCD02950) |
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| Predicted Species Reactivity | Mouse|Mus musculus | ||||||||||||||||||||||
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| Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
| ELISA Kit Duration | 3h | ||||||||||||||||||||||
| ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tryptophan Hydroxylase 2 (TPH2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tryptophan Hydroxylase 2 (TPH2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tryptophan Hydroxylase 2 (TPH2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Tryptophan Hydroxylase 2 (TPH2) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
| ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tryptophan Hydroxylase 2 (TPH2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tryptophan Hydroxylase 2 (TPH2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
| Sample Type | Tissue homogenates, cell lysates and other biological fluids | ||||||||||||||||||||||
| Sensitivity | 0.056 ng/mL | ||||||||||||||||||||||
| Specificity | This assay has high sensitivity and excellent specificity for detection of Tryptophan Hydroxylase 2 (TPH2). No significant cross-reactivity or interference between Tryptophan Hydroxylase 2 (TPH2) and analogues was observed. | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA | ||||||||||||||||||||||
| Protocol Information |
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| Gene Symbol | Tph2 |
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| Gene Full Name | tryptophan hydroxylase 2 |
| Alias Symbols | AU043594;neuronal tryptophan hydroxylase;Ntph;tryptophan 5-hydroxylase 2;tryptophan 5-monooxygenase 2. |
| NCBI Gene Id | 216343 |
| Protein Name | Tryptophan 5-hydroxylase 2 |
| Description of Target | Tryptophan 5-hydroxylase 2 |
| Uniprot ID | Q8CGV2 |
| Protein Accession # | NP_775567.2 |
| Nucleotide Accession # | NM_173391.3 |
| Protein Size (# AA) | 488 |