TNF alpha Polyclonal Antibody
Cat# bs-2081R-20ul
Size : 20ul
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TNF alpha Polyclonal Antibody
Mouse liver lysates probed with TNF alpha Polyclonal Antibody, unconjugated (bs-2081R) at 1:300 overnight at 4\u00b0C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37\u00b0C.
Applications
Reactivity
| Overview | |
| Catalog # | bs-2081R |
| Product Name | TNF alpha Polyclonal Antibody |
| Applications | WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F) |
| Reactivity | Human, Mouse, Rat, Rabbit, Horse |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from mouse TNF-alpha |
| Immunogen Range | 181-235/235 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 21926 |
| Swiss Prot | P06804 |
| Subcellular location | Secreted, Cell membrane |
| Synonyms | DIF; Tnfa; TNF-a; TNFSF2; Tnfsf1a; TNFalpha; TNF-alpha; Tumor necrosis factor; Cachectin; Tumor necrosis factor ligand superfamily member 2; Tnf |
| Background | Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |