TMB PLUS2 Accessory Reagent
Cat# OOSA09609
Size : 100ml
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
| Datasheets/Manuals | Printable datasheet for TMB PLUS2 Accessory Reagent (OOSA09609) |
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| Product Format | Liquid |
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| Application | Enzyme-linked immunosorbent assay |
| Reconstitution and Storage | 2°C to 8°C |
| Application Info | ELISA: Neat |
| Procedure | 1. It is recommended that 100ul of OOSA09609 TMB substrate is used per microtiter well. Pour the desired amount of substrate into a sealed container and allow it to reach room termperature in the dark. 2. Add 100ul substrate solution per microtiter well. 3. Allow development of the substrate solution. Time of development is typically 5-30 minutes. For best results, the plate should be kept in the dark during incubation e.g. wrapped in tinfoil. 4. For kinetic assays, read absorbance at 655nm (blue). For endpoint assays, add an equal volume of 0.2M sulphuric acid and read the absorbance at 450nm (yellow). This endpoint solution is stable for up to one hour. N.B. If reduced intensity is required, it is recommended that the development time is reduced or the antibody/conjugate is diluted further. (Dilution of OOSA09609 is not recommended). |