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> TMB ELISA PEROXIDASE SUBSTRATE

TMB ELISA PEROXIDASE SUBSTRATE

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Cat# TMBE-100

Size : 100mL

Brand : Rockland Immunochemicals

Contact local distributor :


Phone : +1 850 650 7790

Background

TMB (3,3',5,5'-Tetramethylbenzidine) is a colorimetric substrate for peroxidase (HRP)-based enzyme immunoassays, notably ELISAs, and is known for producing a blue color that changes to yellow upon stopping the reaction with sulfuric acid. The color change is quantifiable at 450 nm. TMB's high sensitivity makes it ideal for detecting low concentrations of analytes.

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Specifications for TMB ELISA Peroxidase Substrate

Product Details

TMB ELISA Peroxidase Substrate - TMBE-100
3,3’,5,5’-Tetramethylbenzidine, TMB ELISA substrate, TMBE Substrate, chromogenic ELISA substrate

Target Details

pH: 4.0 +/- 0.2 Stability @ 18° to 26°C: PASS Stability @ 4°C: PASS QC Raw Material: PASS Absorbance check of final product: PASS Performance Data per ELISA: PASS

Application Details

ELISA
TMB ELISA Peroxidase Substrate comes ready to use. No dilutions are required. TMB ELISA Peroxidase Substrate will produce a soluble blue end product read at 370 nm or 655 nm. TMB ELISA Peroxidase Substrate incubation time will vary depending on the assay conditions.

Formulation

Liquid - clear to very light blue colored liquid
1x
Proprietary buffer with enhancer

Shipping & Handling

Ambient
Store container at 4° C prior to opening. Protect from moisture and light. No special shipping conditions or precautions are required.
Expiration date is one (1) year from date of receipt.

References (19)

(). Detection of SARS-CoV-2 peptide-specific antibodies in Syrian hamster serum by ELISA. J Immunol Methods.
Applications
EIA, ELISA
PubMed
(). Assessing the compatibility of primary human hepatocyte culture within porous silk sponges. Rsc Advances
Applications
EIA, ELISA
PubMed
(). Immunological imprint of COVID-19 on human peripheral blood leukocyte populations. Allergy
Applications
E, EIA
PubMed
(). LKB1-PTEN axis controls Th1 and Th17 cell differentiation via regulating mTORC1. J Mol Med (Berl).
Applications
Undefined
PubMed
(). Microscale Collagen and Fibroblast Interactions Enhance Primary Human Hepatocyte Functions in Three-Dimensional Models. Gene Expr.
Applications
E, EIA
PubMed
(). Primary Human Hepatocytes Maintain Long-term Functions in Porous Silk Scaffolds Containing Extracellular Matrix Proteins. bioRxiv
Applications
E, EIA
Source
(). Antibody-secreting cell destiny emerges during the initial stages of B-cell activation. Nat Commun.
Applications
E, EIA
PubMed
(). Serum lipoprotein–derived fatty acids regulate hypoxia-inducible factor. J Biol Chem.
Applications
E, EIA
PubMed
(). Convergent structural features of respiratory syncytial virus neutralizing antibodies and plasticity of the site V epitope on prefusion F. PLoS Pathog.
Applications
E, EIA
PubMed
(). Intra-seasonal antibody repertoire analysis of a subject immunized with an MF59®-adjuvanted pandemic 2009 H1N1 vaccine. Vaccine.
Applications
E, EIA
PubMed
(). The Histone Demethylase LSD1 Regulates B Cell Proliferation and Plasmablast Differentiation. Journal of Immunology (Baltimore, Md. : 1950)
Applications
EIA, ELISA
PubMed
(). Antibodies against adenovirus fiber and penton base proteins inhibit adenovirus vector-mediated transduction in the liver following systemic administration. Scientific Reports
Applications
EIA, ELISA
PubMed
(). B cell activation and plasma cell differentiation are inhibited by de novo DNA methylation. Nature Communications
Applications
EIA, ELISA
PubMed
(). Pre-fusion RSV F strongly boosts pre-fusion specific neutralizing responses in cattle pre-exposed to bovine RSV. Nature Communications
Applications
EIA, ELISA
PubMed
(). Novel antibody probes for the characterization of endosialin/TEM-1. Oncotarget
Applications
EIA, ELISA
PubMed
(). Cytomegalovirus viral load within blood increases markedly in healthy people over the age of 70 years. Immunity & Ageing : I & A
Applications
EIA, ELISA
PubMed
(). Antigenic Characterization of the HCMV gH/gL/gO and Pentamer Cell Entry Complexes Reveals Binding Sites for Potently Neutralizing Human Antibodies. PLOS Pathogens
Applications
E, EIA
PubMed
(). Suppression of leaky expression of adenovirus genes by insertion of microRNA-targeted sequences in the replication-incompetent adenovirus vector genome. Molecular Therapy. Methods & Clinical Development
Applications
EIA, ELISA
PubMed
(). Characterization of the human folate receptor alpha via novel antibody-based probes. Oncotarget
Applications
EIA, ELISA
PubMed

Related Protocols

ELISA Protocol
Sandwich ELISA Protocol for Collagen

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Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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