TIMP3 Chemi-Luminescent ELISA Kit (Horse)
Cat# OKCD03965
Size : 96Wells
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
| Datasheets/Manuals | Printable datasheet for TIMP3 Chemi-Luminescent ELISA Kit (Horse) (OKCD03965) |
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| Predicted Species Reactivity | Equus caballus|Horse | ||||||||||||||||||||||
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| Application | Chemiluminescent immunoassay | ||||||||||||||||||||||
| ELISA Kit Detection Method | Chemiluminescent | ||||||||||||||||||||||
| ELISA Kit Duration | 2h, 40min | ||||||||||||||||||||||
| ELISA Kit Linearity |
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| ELISA Kit Principle | The microplate provided in this kit has been pre-coated with an antibody specific to Tissue Inhibitors Of Metalloproteinase 3 (TIMP3). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Tissue Inhibitors Of Metalloproteinase 3 (TIMP3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) level in the sample or standard. | ||||||||||||||||||||||
| ELISA Kit Range | 27.4-20,000pg/mL | ||||||||||||||||||||||
| ELISA Kit Recovery |
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| ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
| Sample Type | Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids | ||||||||||||||||||||||
| Sensitivity | 0.312 ng/mL | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | TIMP3 |
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| Gene Full Name | TIMP metallopeptidase inhibitor 3 |
| Alias Symbols | metalloproteinase inhibitor 3;TIMP-3;tissue inhibitor of metalloproteinase 3;tissue inhibitor of metalloproteinases 3. |
| NCBI Gene Id | 100033947 |
| Protein Name | Metalloproteinase inhibitor 3 |
| Description of Target | Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli. |
| Uniprot ID | Q9TUL9 |
| Protein Accession # | NP_001075339 |
| Nucleotide Accession # | NM_001081870 |