THBS1 ELISA Kit (Monkey)
Cat# OKBB00840
Size : 96wells/kit
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
THBS1 ELISA Kit (Monkey) (OKBB00840)
| Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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| Predicted Species Reactivity | Monkey | ||||||||||||||||||||||||||||||||||||||||||
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| Application | ELISA | ||||||||||||||||||||||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||||||||||||||||||||||
| ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||||||||||||||||||||||
| ELISA Kit Principle | Aviva's Monkey THBS1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for THBS1 has been precoated onto 96-well plates. Standards(NS0, N19-P1170) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for THBS1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the Monkey THBS1 amount of sample captured in plate. | ||||||||||||||||||||||||||||||||||||||||||
| ELISA Kit Range | 1.56 ng/ml - 100 ng/ml | ||||||||||||||||||||||||||||||||||||||||||
| ELISA Kit Reproducibility |
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| ELISA Kit Component |
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| Additional Information | To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
*number of samples for each test n=16. | ||||||||||||||||||||||||||||||||||||||||||
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| Reconstitution and Storage | Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.) | ||||||||||||||||||||||||||||||||||||||||||
| Sample Type | Sandwich High Sensitivity ELISA kit for Quantitative Detection of monkey primate THBS1/TSP1 | ||||||||||||||||||||||||||||||||||||||||||
| Sensitivity | <50 pg/ml | ||||||||||||||||||||||||||||||||||||||||||
| Immunogen | Expression system for standard: NS0; Immunogen sequence: N19-P1170 | ||||||||||||||||||||||||||||||||||||||||||
| Gene Symbol | THBS1 |
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| Gene Full Name | Thrombospondin-1 |
| Alias Symbols | Thrombospondin-1;THBS1;TSP, TSP1; |
| NCBI Gene Id | 705413 |
| Protein Name | Thrombospondin-1 |
| Description of Target | Thrombospondin 1, also known as THBS1, is a protein that in humans in encoded by the THBS1 gene. Thrombospondin 1 is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. By in situ hybridization, the THBS1 gene was mapped to monkey primate 15q15 and the cognate gene to monkey primate chromosome 2(region F) and was localized to 15q11-qter by Southern analysis of human-rodent somatic cell hybrids. Thrombospondin I is a multimodular secreted protein that associates with the extracellular matrix and possesses a variety of biologic functions, including a potent antiangiogenic activity. Other thrombospondin genes include thrombospondins II, III, and IV. |
| Uniprot ID | F6WIY2 |
| Protein Accession # | NP_003237.2 |
| Nucleotide Accession # | NM_003246.3 |