Tetracyclines ELISA Kit
Cat# OKAO00129
Size : 96Wells
Brand : Aviva Systems Biology
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Phone : +1 850 650 7790
| Datasheets/Manuals | Printable datasheet for Tetracyclines ELISA Kit (OKAO00129) |
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| Predicted Species Reactivity | General | ||||||||||||||||||||||
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| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~4 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology Tetracyclines ELISA Kit (OKAO00129) is based on standard competitive inhibition enzyme-linked immuno-sorbent assay technology. Tetracyclines has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells along with a rabbit anti-Tetracyclines antibody and an anti-rabbit / HRP Conjugated antibody, then incubated. Wells are washed and detection substrates are added and incubated. An enzymatic reaction is produced through the addition of TMB which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm which is quantitatively proportional to the amount of detector antibody bound in the well and inversely proportional to the amount of Sulfadimethoxine in the sample. | ||||||||||||||||||||||
| ELISA Kit Range | 0.05-4.05 ppb | ||||||||||||||||||||||
| ELISA Kit Recovery |
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| ELISA Kit Reproducibility | Mean Intra-Assay: CV<8% (n= 3 x 20) Mean Inter-Assay: CV<12% (n= 3 x 40) | ||||||||||||||||||||||
| ELISA Kit Component |
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| Additional Information | CAS: 60-54-8 PubChem: 54675776 | ||||||||||||||||||||||
| :: | Molecular Formula: C22H24N2O8 | ||||||||||||||||||||||
| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Tissue (Including aquatic products), Honey, Milk, Egg, Water. | ||||||||||||||||||||||
| Sensitivity |
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| Specificity |
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| Assay Info | Assay Methodology: Competitive Inhibition ELISA |
| Alias Symbols | TET, Tetracycline, Deschlorobiomycin, Tetracyclinum, Achromycin, 60-54-8, Tetracyclin |
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| Protein Name | Tetracyclines |
| Description of Target | Tetracycline is a broad-spectrum naphthacene antibiotic produced semisynthetically from chlortetracycline, an antibiotic isolated from the bacterium Streptomyces aureofaciens. In bacteria, tetracycline binds to the 30S ribosomal subunit, interferes with the binding of aminoacyl-tRNA to the mRNA-ribosome complex, thereby inhibiting protein synthesis. |
| Molecular Weight | 444.43456 g/mol |