Specifications

Product Data
Applications	IF, IHC, WB
Recommended Dilution	WB 1:500-1:2,000; IF 1:50-1:500; IHC (paraffin) 1:50-1:500; IHC (frozen) 1:50-1:500
Target	tdTomato tag
Host	Goat
Clonality	Polyclonal
Immunogen	Purified recombinant peptide produced in E. coli.
Isotype	IgG
Formulation	Polyclonal antibody supplied as a 200 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide.
Concentration	3mg/ml
Purification	epitope affinity purified
Conjugation	Unconjugated
Background	Red Fluorescent Protein
Note	In 293HEK cells transfected with cds plasmid detects a band of 55 kDa by Western blot. It also detects tdTomato in brain sections by IHC. This antibody (AB8181) is specific for tdTomato and mCherry proteins. It does not cross-react to GFP (green fluorescent protein).