SUV39H1 (Suppressor Of Variegation 3-9 Homolog 1, Histone H3-K9 Methyltransferase 1, H3-K9-HMTase 1, Su(var)3-9 Homolog 1, KMT1A, Histone-lysine N-methyltransferase SUV39H1, Position-effect Variegation 3-9 Homolog, Lysine N-methyltransferase 1A) (MaxLight 550)

Cat# S8950-01A-ML550-100ul

Size : 100ul

Brand : US Biological

Contact local distributor :


Phone : +1 850 650 7790


Host
mouse

Conjugate
MaxLight™550

Isotype
IgG1

Clone Number
42AT239.96.72

Grade
Affinity Purified

Applications
FLISA WB

Shipping Temp
Blue Ice

Storage Temp
4°C Do Not Freeze

MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.

Similar to acetylation and phosphorylation, histone methylation at the N-terminal tail has emerged as an important role in regulating chromatin dynamics and gene activity. Histone methylation occurs on arginine and lysine residues and is catalyzed by two families of proteins, the protein arginine methyltransferase family and the SET-domain-containing methyltransferase family. Five members have been identified in the arginine methyltransferase family. About 27 are grouped into the SET-domain family, and another 17 make up the PR domain family that is related to the SET domain family. The retinoblastoma protein-interacting zinc finger geneRIZ1 is a tumor suppressor gene and a FOUNDING member of the PR domain family. RIZ1 inactivation is commonly found in many types of human cancers and occurs through loss of mRNA expression, frame shift mutation, chromosomal deletion, and missense mutation. RIZ1 is also a tumor susceptibility gene in mice. The loss of RIZ1 mRNA in human cancers was shown to associate with DNA methylation of its promoter CpG island. Methylation of the RIZ1 promoter strongly correlated with lost or decreased RIZ1 mRNA expression in breast, liver, colon, and lung cancer cell lines as well as in liver cancer tissues.

Applications:
Suitable for use in FLISA and Western Blot. Other applications not tested.

Recommended Dilution:
FLISA: 1:1,000
Western Blot: 1:100-1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Product Type
Mab

Isotype
IgG1

Clone No
42AT239.96.72

Host
mouse

Source
human

Concentration
As reported

Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.

Purity
Purified by Protein G affinity chromatography.

Immunogen
Purified recombinant GST fusion protein encoding N-terminal of human SUV39H1.

Specificity
Recognizes human SUV39H1. Species Crossreactivity


Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.