Spi1 (NM_011355) Mouse Tagged ORF Clone
CAT#: MR203632
- TrueORF®
Sfpi1 (Myc-DDK-tagged) - Mouse SFFV proviral integration 1 (Sfpi1)
ORF Plasmid: tGFP
Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro
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Product Images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Symbol | Spi1 |
| Synonyms | Dis-1; Dis1; PU.1; Sfpi-1; Sfpi1; Spi-1; Tcfpu1; Tfpu.1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | >MR203632 representing NM_011355 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTACAGGCGTGCAAAATGGAAGGGTTTTCCCTCACCGCCCCTCCATCGGATGACTTGGTTACTTACG ATTCAGAGCTATACCAACGTCCAATGCATGACTACTACTCCTTCGTGGGCAGCGATGGAGAAAGCCATAG CGATCACTACTGGGATTTCTCCGCACACCATGTCCACAACAACGAGTTTGAGAACTTCCCTGAGAACCAC TTCACAGAGCTGCAGAGTGTGCAGCCCCCGCAGCTACAGCAGCTCTATCGCCACATGGAGCTGGAACAGA TGCACGTCCTCGATACTCCCATGGTGCCACCCCACACCGGCCTCAGTCACCAGGTTTCCTACATGCCCCG GATGTGCTTCCCTTATCAAACCTTGTCCCCAGCCCACCAGCAGAGCTCAGATGAGGAGGAGGGTGAGAGG CAGAGCCCTCCCCTGGAGGTGTCTGATGGAGAAGCTGATGGCTTGGAGCCTGGGCCAGGTCTTCTGCACG GGGAGACAGGCAGCAAGAAAAAGATTCGCCTGTACCAGTTCCTGCTGGACCTGCTGCGCAGCGGCGACAT GAAGGACAGCATCTGGTGGGTGGACAAGGACAAAGGTACCTTCCAGTTCTCGTCCAAGCACAAGGAGGCG CTGGCGCACCGCTGGGGCATCCAGAAGGGCAACCGCAAGAAGATGACCTACCAGAAGATGGCGCGCGCGC TGCGCAACTACGGCAAGACAGGCGAGGTGAAGAAAGTCAAGAAGAAGCTCACCTACCAGTTCAGCGGCGA GGTGCTGGGCCGTGGGGGCCTGGCCGAGCGGCGCCTCCCGCCCCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR203632 representing NM_011355 Red=Cloning site Green=Tags(s) MLQACKMEGFSLTAPPSDDLVTYDSELYQRPMHDYYSFVGSDGESHSDHYWDFSAHHVHNNEFENFPENH FTELQSVQPPQLQQLYRHMELEQMHVLDTPMVPPHTGLSHQVSYMPRMCFPYQTLSPAHQQSSDEEEGER QSPPLEVSDGEADGLEPGPGLLHGETGSKKKIRLYQFLLDLLRSGDMKDSIWWVDKDKGTFQFSSKHKEA LAHRWGIQKGNRKKMTYQKMARALRNYGKTGEVKKVKKKLTYQFSGEVLGRGGLAERRLPPH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
| ACCN | NM_011355 |
| ORF Size | 816 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at info@biotrend.com The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011355.2 |
| RefSeq Size | 1333 bp |
| RefSeq ORF | 819 bp |
| Locus ID | 20375 |
| UniProt ID | P17433 |
| Cytogenetics | 2 50.44 cM |
| MW | 31.8 kDa |
| Gene Summary | Binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. Also binds RNA and may modulate pre-mRNA splicing.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | |
|---|---|---|---|
| MC200585 | Sfpi1 (untagged) - Mouse SFFV proviral integration 1 (Sfpi1), (10ug) | 450,00 EUR | |
| MG203632 | Sfpi1 (tGFP-tagged) - Mouse SFFV proviral integration 1 (Sfpi1) | 650,00 EUR | |
| MR203632L1 | Lenti ORF clone of Sfpi1 (Myc-DDK-tagged) - Mouse SFFV proviral integration 1 (Sfpi1) | 750,00 EUR | |
| MR203632L2 | Lenti ORF clone of Sfpi1 (mGFP-tagged) - Mouse SFFV proviral integration 1 (Sfpi1) | 750,00 EUR | |
| MR203632L3 | Lenti ORF clone of Sfpi1 (Myc-DDK-tagged) - Mouse SFFV proviral integration 1 (Sfpi1) | 750,00 EUR | |
| MR203632L4 | Lenti ORF clone of Sfpi1 (mGFP-tagged) - Mouse SFFV proviral integration 1 (Sfpi1) | 750,00 EUR |