SMAD1 ELISA Kit (Bovine)
Cat# OKEH07443
Size : 96T
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
SMAD1 ELISA Kit (Bovine) (OKEH07443)
| Datasheets/Manuals | Printable datasheet for SMAD1 ELISA Kit (Bovine) (OKEH07443) |
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| Predicted Species Reactivity | Bos taurus|Bovine | ||||||||||||||||||||||
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| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology SMAD1 ELISA Kit (Bovine) (OKEH07443) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SMAD1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SMAD1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SMAD1 captured in well. | ||||||||||||||||||||||
| ELISA Kit Range | 78-5000pg/mL | ||||||||||||||||||||||
| ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102% (n = 20) | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Mean Intra-assay CV%: <=6.1% (n = 20) Mean Inter-assay CV%: <=7.5% (n = 20) | ||||||||||||||||||||||
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| Additional Information | Function: Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). May act synergistically with SMAD4 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. | ||||||||||||||||||||||
| :: | Subcellular Location: Cytoplasm Nucleus Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Colocalizes with LEMD3 at the nucleus inner membrane (By similarity). Forms a ternary complex with PSMB4 and OAZ1 before PSMB4 is incorporated into the 20S proteasome (By similarity). | ||||||||||||||||||||||
| :: | Unigene: Bt.37151 SMR: Q1JQA2 String: 9913.ENSBTAP00000003668 | ||||||||||||||||||||||
| :: | KEGG: bta:540488 Pfam: PF03165 | ||||||||||||||||||||||
| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sensitivity | 36pg/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant Bovine Mothers against decapentaplegic homolog 1 | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | SMAD1 |
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| Gene Full Name | SMAD family member 1 |
| Alias Symbols | MAD homolog 1, mad-related protein 1, mothers against decapentaplegic homolog 1, mothers against DPP homolog 1, Sma- and Mad-related protein 1, SMAD 1, SMAD family member 1, SMAD, mothers against DPP homolog 1. |
| NCBI Gene Id | 540488 |
| Protein Name | Mothers against decapentaplegic homolog 1 |
| Description of Target | Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). May act synergistically with SMAD4 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. |
| Uniprot ID | Q1JQA2 |
| Protein Accession # | NP_001069691.2 |
| Nucleotide Accession # | NM_001076223.2 |