Anti-RFP (GOAT) Antibody

Cat# 200-101-379

Size : 100ug

Brand : Rockland Immunochemicals

Contact local distributor :


Phone : +1 850 650 7790

Background

Antibodies to RFP (Discosoma spp.) are intended for use in immunological assays including ELISA, western blotting, immunofluorescence, and fluorescence activated cell sorting (FACS).

Expand Background

Specifications for RFP Antibody

Product Details

Anti-RFP (GOAT) Antibody - 200-101-379
goat anti-RFP antibody, DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583
Goat
Polyclonal
IgG

Target Details

DsRed  - View All DsRed Products
RFP, rRFP
Recombinant Protein
The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
Anti-RFP (GOAT) is an IgG fraction purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Red Fluorescent Protein (Discosoma). No reaction was observed against Human, Mouse or Rat serum proteins.   Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry.
UniProtKB - Q9U6Y8

Application Details

WB
EM, FC, IF, IHC, Multiplex  - View References
Polyclonal anti-RFP has been tested by and western blot and is designed to detect RFP and its variants. Optimal titers for applications should be determined by the researcher. Expect a band ~27kDa in Western Blot in the appropriate cell lysate or extract.

Formulation

Liquid (sterile filtered)
1 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None

Shipping & Handling

Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.

References (26)

(). Generation of a CRF1-Cre transgenic rat and the role of central amygdala CRF1 cells in nociception and anxiety-like behavior. ELife
Applications
IF, Confocal Microscopy
PubMed
(). Edinger-Westphal peptidergic neurons enable maternal preparatory nesting. Neuron
Applications
IHC, ICC, Histology
PubMed
(). Saccharin Stimulates Insulin Secretion Dependent on Sweet Taste Receptor-Induced Activation of PLC Signaling Axis. Biomedicines
Applications
IF, Confocal Microscopy
PubMed
(). Saccharin Stimulates Insulin Secretion Dependent on Sweet Taste Receptor-Induced Activation of PLC Signaling Axis. Biomedicines.
Applications
IF, Confocal Microscopy
Source
(). Inhibitory Fear Memory Engram in the Mouse Central Lateral Amygdala. Cell Press Preprint
Applications
Undefined
Source
(). Meflin defines mesenchymal stem cells and/or their early progenitors with multilineage differentiation capacity. Genes Cells.
Applications
IF, Confocal Microscopy
PubMed
(). Wnt-modified materials mediate asymmetric stem cell division to direct human osteogenic tissue formation for bone repair. Nat Mater.
Applications
Undefined
PubMed
(). Pluripotency state regulates cytoneme selectivity and self-organization of embryonic stem cells. J Cell Biol.
Applications
IF, Confocal Microscopy
PubMed
(). Targeting KDM4A epigenetically activates tumor-cell-intrinsic immunity by inducing DNA replication stress. Mol Cell.
Applications
Undefined
PubMed
(). mGlu1 potentiation enhances prelimbic somatostatin interneuron activity to rescue schizophrenia-like physiological and cognitive deficits. Cell Rep.
Applications
IF, Confocal Microscopy
PubMed
(). Wolframin-1–expressing neurons in the entorhinal cortex propagate tau to CA1 neurons and impair hippocampal memory in mice. Sci Transl Med.
Applications
IHC, ICC, Histology; Immuno-EM
PubMed
(). Generation and validation of a CRF1:Cre transgenic rat: The role of central amygdala CRF1 in nociception and anxiety-like behavior. bioRxiv Preprint
Applications
IF, Confocal Microscopy
Source
(). Neuroblastoma differentiation in vivo excludes cranial tumors. Dev Cell.
Applications
Undefined
PubMed
(). Fibrosis Without Myofibroblasts Revealed by Genetic Analysis of PDGFRα. Cell Press Preprint
Applications
Undefined
Source
(). Astrocytes regulate ultra-slow arteriole oscillations via stretch-mediated TRPV4-COX-1 feedback. Cell Rep.
Applications
IHC, ICC, Histology
PubMed
(). Origin, specification and differentiation of a rare supporting-like lineage in the developing mouse gonad. HAL Preprint.
Applications
IHC, ICC, Histology
Source
(). Spatiotemporal dynamics of PIEZO1 localization controls keratinocyte migration during wound healing. Elife.
Applications
IF, Confocal Microscopy
PubMed
(). In vitro reconstitution of the whole male germ-cell development from mouse pluripotent stem cells. Cell Stem Cell.
Applications
Undefined
PubMed
(). A Specific Neuroligin3-αNeurexin1 Code Regulates GABAergic Synaptic Function in Mouse Hippocampus. ELife
Applications
IF, Confocal Microscopy
PubMed
(). Krüppel-like Factor 5 Regulates Stemness, Lineage Specification, and Regeneration of Intestinal Epithelial Stem Cells. Cell Mol Gastroenterol Hepatol.
Applications
IF, Confocal Microscopy; FC, FACS, FLOW
PubMed
(). Stromal CCL2 Signaling Promotes Mammary Tumor Fibrosis through Recruitment of Myeloid-Lineage Cells. Cancers (Basel).
Applications
IF, Confocal Microscopy
PubMed
(). Vasoactive Intestinal Polypeptide-Expressing Interneurons in the Hippocampus Support Goal-Oriented Spatial Learning. Neuron.
Applications
IHC, ICC, Histology
PubMed
(). A Mac2-positive progenitor-like microglial population survives independent of CSF1R signaling in adult mouse brain. Elife.
Applications
IHC, ICC, Histology
PubMed
(). Acute inflammation regulates neuroregeneration through the NF-κB pathway in olfactory epithelium. Proc Natl Acad Sci U S A.
Applications
IHC, ICC, Histology
PubMed
(). Comparative Genomic Analysis of Drechmeria coniospora Reveals Core and Specific Genetic Requirements for Fungal Endoparasitism of Nematodes. PloS Genetics
Applications
WB, IB, PCA
PubMed
(). Regulated delivery of molecular cargo to invasive tumour-derived microvesicles. Nature Communications
Applications
WB, IB, PCA
PubMed

Related Protocols

Adherent Cell Lysis Protocol
Flow Cytometry (FC) Protocol
Fluorescent Western Blotting Protocol
Heat-induced Antigen Retrieval Protocol
Histone Immunoblotting Protocol
Immunocytochemistry (ICC) Protocol
Immunofluorescence (IF) Protocol
Immunohistochemistry (IHC) Protocol
In-Cell Western (ICW) Protocol
IP-WB with TrueBlot® Protocol
Multi-Lysate Western Blotting Protocol
Nuclear & Cytoplasmic Extract Protocol
Protease-induced Antigen Retrieval Protocol
Staining Paraffin Sections by PAP Procedure
Suspension Cultured Cell Lysis Protocol
Western Blotting (WB) Protocol

Certificate of Analysis Lookup

Invalid lot number

Search

If you need help finding your CoA, contact us

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Related products to: RFP Antibody

Related Resources

You might also be interested by the following products:



Cat#
Description
Cond.
Price Bef. VAT
DS-MB-01410
 0.1ml 
PAB27987
 100uL 
DS-PB-00926
 0.1mg