RBP3 ELISA Kit (Bovine)
Cat# OKCA01036
Size : 96Wells
Brand : Aviva Systems Biology
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Bovine | ||||||||||||||||||||||
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ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~ 3.5 Hours | ||||||||||||||||||||||
ELISA Kit Linearity |
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ELISA Kit Principle | Aviva Systems Biology RBP3 ELISA Kit (Bovine) (OKCA01036) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for RBP3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for RBP3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample RBP3 captured in the well. | ||||||||||||||||||||||
ELISA Kit Range | 0.156 - 10 ng/mL | ||||||||||||||||||||||
ELISA Kit Recovery |
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ELISA Kit Reproducibility | Intra-assay Precision: 3 samples with known low, middle and high level OKCA01036 were tested with 20 replicates across one plate. Inter-assay Precision: 3 samples with known low, middle and high level OKCA01036 were tested on three different plates with 8 replicates, respectively. Intra-Assay CV: <8% Inter-Assay CV: <10% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, Plasma, Tissue Homogenates | ||||||||||||||||||||||
Sensitivity | 0.039 ng/mL | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sanadwich ELISA | ||||||||||||||||||||||
Protocol Information |
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Gene Symbol | RBP3 |
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Gene Full Name | Retinol-binding protein 3 |
Alias Symbols | D10S64, D10S65, D10S66, IRBP, RBPI, interphotoreceptor retinoid-binding protein|retinol-binding protein 3|retinol-binding protein 3, interstitial |
NCBI Gene Id | 281443 |
Protein Name | Retinol-binding protein 3 |
Description of Target | IRBP shuttles 11-cis and all trans retinoids between the retinol isomerase in the pigment epithelium and the visual pigments in the photoreceptor cells of the retina. |
Uniprot ID | P12661 |
Protein Accession # | NP_776589.1 |
Nucleotide Accession # | NM_174164.1 |