Rat IgA + IgG + IgM (H+L chain) Goat Polyclonal Antibody
CAT#: R1377HRP
Rat IgA + IgG + IgM (H+L chain) goat polyclonal antibody, HRP
Conjugation: Unconjugated Biotin FITC
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Specifications
Product Data | |
Applications | ELISA, IHC, WB |
Recommended Dilution | ELISA: 1/100,000. Western blot: 1/500-1/3,000. Immunohistochemistry: 1/500-1/1,500. |
Reactivities | Rat |
Host | Goat |
Clonality | Polyclonal |
Immunogen | Rat IgG, IgA, IgM whole molecules |
Specificity | This product was prepared from polyspecific antiserum by immunoaffinity chromatography using Rat antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum. This reagent is suitable for the detection of all Rat immunoglobulin isotypes and chain combinations. |
Formulation | 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 Label: HRP State: Lyophilized purified IgG fraction Stabilizer: 10 mg/ml BSA (IgG and Protease free) Preservative: 0.01% (w/v) Thimerosal Label: Horseradish Peroxidase. Conjugation Chemistry: Periodate |
Reconstitution Method | Restore with 1.0 ml of deionized water (or equivalent). |
Concentration | 2.0 mg/ml (by UV absorbance at 280 nm) |
Purification | Immunoaffinity Chromatography |
Conjugation | HRP |
Storage | Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. |
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