Rat CTSL (Cathepsin L) CLIA Kit

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Chemiluminescence

Gene Symbol:	Ctsl
Gene ID:	25697
Uniprot ID:	CATL1_RAT
Specificity:	This kit recognizes Rat CTSL in samples. No significant cross-reactivity or interference between Rat CTSL and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Tissue Specificity	Both mature cathepsin L1 and procathepsin L are found in the upper epidermis. The lower epidermis predominantly contains procathepsin L. In seminiferous tubules expression is greater at stages VI-VII than at stages IX-XII.
Post Translational Modifications	During export along the endocytic pathway, pro-CTSL undergoes several proteolytic cleavages to generate the CTSL single-chain and two-chain mature forms, composed of a heavy chain linked to a light chain by disulfide bonds. Autocleavage.produces the single-chain CTSL after cleavage of the propeptide. The cleavage can be intermolecular.
Function	Thiol protease important for the overall degradation of proteins in lysosomes. Plays a critical for normal cellular functions such as general protein turnover, antigen processing and bone remodeling. Involved in the solubilization of cross-linked TG/thyroglobulin and in the subsequent release of thyroid hormone thyroxine (T4) by limited proteolysis of TG/thyroglobulin in the thyroid follicle lumen. In neuroendocrine chromaffin cells secretory vesicles, catalyzes the prohormone proenkephalin processing to the active enkephalin peptide neurotransmitter. In thymus, regulates CD4(+) T cell positive selection by generating the major histocompatibility complex class II (MHCII) bound peptide ligands presented by cortical thymic epithelial cells. Also mediates invariant chain processing in cortical thymic epithelial cells. Major elastin-degrading enzyme at neutral pH. Accumulates as a mature and active enzyme in the extracellular space of antigen presenting cells (APCs) to regulate degradation of the extracellular matrix in the course of inflammation. Secreted form generates endostatin from COL18A1. Critical for cardiac morphology and function. Plays an important role in hair follicle morphogenesis and cycling, as well as epidermal differentiation. Required for maximal stimulation of steroidogenesis by TIMP1.
Protein Name	Procathepsin L Cathepsin L1 Cyclic Protein 2 Cp-2 Major Excreted Protein Mep Cleaved Into - Cathepsin L - Cathepsin L Heavy Chain - Cathepsin L Light Chain
Database Links	Reactome: R-RNO-1442490 Reactome: -RNO-1679131 Reactome: -RNO-2022090 Reactome: -RNO-2132295 Reactome: -RNO-8939242
Cellular Localisation	Lysosome Apical Cell Membrane Peripheral Membrane Protein Extracellular Side Cytoplasmic Vesicle Secretory Vesicle Chromaffin Granule Secreted Extracellular Space Localizes To The Apical Membrane Of Thyroid Epithelial Cells Released At Extracellular Space By Activated Dendritic Cells And Macrophages
Alternative CLIA Names	Procathepsin L CLIA kit Cathepsin L1 CLIA kit Cyclic Protein 2 CLIA kit Cp-2 CLIA kit Major Excreted Protein CLIA kit Mep Cleaved Into - Cathepsin L - Cathepsin L Heavy Chain - Cathepsin L Light Chain CLIA kit Ctsl CLIA kit Ctsl1 CLIA kit
Specificity	This kit recognizes Rat CTSL in samples. No significant cross-reactivity or interference between Rat CTSL and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	102-116	108
EDTA plasma(n=8)	88-103	94
Cell culture media(n=8)	99-115	106

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	378.31	974.94	2718.88	340.48	893.10	2887.50
Standard deviation	42.37	84.14	309.14	33.61	84.84	310.98
CV (%)	11.20	8.63	11.37	9.87	9.50	10.77

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance