Rabep1 (NM_019400) Mouse Tagged ORF Clone

CAT#: MR217501

  • TrueORF®

Rabep1 (Myc-DDK-tagged) - Mouse rabaptin, RAB GTPase binding effector protein 1 (Rabep1)

ORF Plasmid: DDK tGFP

Lentiviral Particles: DDK w/ Puro mGFP w/ Puro

Datasheet
SDS
Plasmid Map

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Product Images

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Symbol Rabep1
Synonyms Rab5ep; rabaptin-5; rabaptin-5alpha
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR217501 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGCAGCCGGGCCCGGCTCCCCAGCCTGACGTTTCTCTTCAGCAACGGGTAGCAGAATTGGAAAAAA
TTAATGCAGAATTTTTACGTGCACAGCAGCAGCTTGAGCAAGAATTTAATCAAAAGAGAGCAAAATTTAA
GGAGCTCTATTTGGCTAAAGAGGAGGATCTGAAGAGGCAAAATGCAGTATTGCAAGCTGCACAGGACGAT
TTGGGTCACCTTCGGACACAGCTGTGGGAGGCTCAGGCAGAGATGGAGAACATTAAGGCAATTGCCACAG
TCTCTGAGAATACTAAGCAAGAAGCTATAGATGAAGTTAAAAGGCAATGGAGAGAAGAAGTTGCTTCACT
TCAGGCTATAATGAAAGAGACAGTCCGTGACTATGAGCATCAGTTTCACCTGAGGCTGGAGCAGGAGCGA
GCACAGTGGGCACAGTATCGAGAATCTGCAGAGCGGGAAATAGCTGACTTAAGAAGAAGGTTGTCTGAAG
GTCAAGAGGAAGAAAATTTAGAAAATGAAATGAAAAAGGCCCAAGAGGATGCTGAAAAGCTTCGTTCTGT
CGTGATGCCCATGGAGAAGGAAATTGCAGCTCTGAAAGATAAACTGACAGAAGCTGAAGACAAGATTAAA
GAGCTGGAGGCCTCAAAGGTTAAGGAGCTGAATCATTATCTGGAGGCCGAGAAGTCTTGCAGGACTGACC
TGGAGATGTACGTAGCTGTTTTGAACACTCAGAAATCTGTGCTACAGGAAGATGCTGAGAAATTGAGGAA
AGAACTGCATGAAGTTTGCCATCTCTTGGAACAAGAACGACAACAACACAACCAGTTAAAGCATACATGG
CAGAAGGCCAACGACCAGTTTCTGGAATCTCAGCGTTTGCTGATGAGAGACATGCAGAGAATGGAGATTG
TGTTAACTTCGGAACAGCTCCGACAAGTTGAAGAACTAAAGAAGAAAGACCAGGAGGAGGATGAACAACA
AAGGGTCAATAAGAGAAAAGATAACAAGAAAACAGATACCGAAGAAGAAGTAAAAATACCAGTAGTGTGT
GCTTTAACTCAAGAAGAATCTTCAACCCCATTATCAAATGAAGAGGAGCATTTAGACAGCACCCATGGGT
CAGTCCATTCCTTGGATGCAGACTTGATGTTACCATCTGGAGATCCATTTAGTAAATCGGACAATGACAT
GTTTAAAGATGGACTCAGGAGAGCTCAGTCTACAGACAGCTTGGGAACCTCAAGCTCATTGCAATCCAAA
GCTTTAGGCTATAACTACAAAGCGAAATCTGCTGGAAATTTGGATGAGTCGGATTTTGGACCACTGGTTG
GAGCAGATTCTGTGTCTGAGAACTTTGATACTGTGTCCCTTGGGTCACTGCAGATGCCAAGTGGATTTAT
GTTAACCAAAGATCAAGAAAGAGCAATTAAGGCAATGACACCTGAGCAGGAGGAGACAGCATCCCTCCTC
TCCAGTGTCACCCAGGGTATGGAGAGTGCATATGTATCTCCCAGTGGTTACCGCTTAGTGAGTGAGACGG
AATGGAATCTCCTGCAGAAAGAGGTACATAATGCTGGAAATAAGCTTGGTAGACGTTGTGATATGTGTTC
CAATTATGAAAAACAGTTACAAGGAATTCAGATTCAGGAAGCTGAAACAAGAGACCAGGTGAAAAAACTG
CAGTTAATGCTCAGGCAAGCTAATGACCAGCTTGAGAAGACGATGAAAGAGAAGCAGGAGCTGGAGGACT
TCCTCAAGCAGAGTGCGGAGGACTCAAGCCACCAGATATCTGCACTTGTTTTAAGAGCCCAAGCCTCTGA
GGTCTTACTTGAAGAGTTACAACAGAGCTTTTCCCAAGCAAAGAGGGACGTTCAGGAGCAGATGGCGGTG
CTGATGCAGTCACGTGAACAGGTTTCAGAAGAGTTGGTGAGGTTACAGAAAGACAACGACAGCCTCCAGG
GGAAGCACAGCTTGCATGTGTCATTACAGCTAGCAGAAGACTTCATTCTTCCAGACACTGTGGAGGTGCT
CCGGGAACTGGTTTTAAAATATCGTGAGAACATTGTTCATGTGCGCACAGCAGCAGACCACATGGAAGAG
AAGCTGAAGGCGGAAATACTTTTCCTAAAAGAGCAAATTCAAGCGGAACAGTGTTTAAAAGAAAACCTGG
AAGAGACACTACAGCTGGAAATAGAAAACTGCAAGGAGGAAATAGTCCTAGCTTCCATTTCTAGTCTAAA
AGCTGAATTAGAAAGAATAAAGGTTGAAAAGGGACAGTTGGAATCTACATTAAGAGAGAAGTCGCAACAA
CTTGAGAGTCTCCAAGAAATGAAAGTTAATTTAGAGGAACAGTTAAAGAAAGAAACTGCTGCTAAGGCTA
CTGTTGAACAGCTCATGTTTGAAGAGAAGAACAAAGCTCAGAGGTTGCAGACAGAATTAGATGTCAGTGA
ACAAGTACAGAGAGATTTTGTAAAACTTTCTCAGACTCTTCAGGTGCAGTTGGAGAGGATCCGGCAAGCA
GACTCCTTGGAGAGGATCCGGGCTATTCTGAATGATACCAAACTGACAGACATTAACCAGCTCCCTGAGA
CA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
>MR217501 protein sequence
Red=Cloning site Green=Tags(s)

MAQPGPAPQPDVSLQQRVAELEKINAEFLRAQQQLEQEFNQKRAKFKELYLAKEEDLKRQNAVLQAAQDD
LGHLRTQLWEAQAEMENIKAIATVSENTKQEAIDEVKRQWREEVASLQAIMKETVRDYEHQFHLRLEQER
AQWAQYRESAEREIADLRRRLSEGQEEENLENEMKKAQEDAEKLRSVVMPMEKEIAALKDKLTEAEDKIK
ELEASKVKELNHYLEAEKSCRTDLEMYVAVLNTQKSVLQEDAEKLRKELHEVCHLLEQERQQHNQLKHTW
QKANDQFLESQRLLMRDMQRMEIVLTSEQLRQVEELKKKDQEEDEQQRVNKRKDNKKTDTEEEVKIPVVC
ALTQEESSTPLSNEEEHLDSTHGSVHSLDADLMLPSGDPFSKSDNDMFKDGLRRAQSTDSLGTSSSLQSK
ALGYNYKAKSAGNLDESDFGPLVGADSVSENFDTVSLGSLQMPSGFMLTKDQERAIKAMTPEQEETASLL
SSVTQGMESAYVSPSGYRLVSETEWNLLQKEVHNAGNKLGRRCDMCSNYEKQLQGIQIQEAETRDQVKKL
QLMLRQANDQLEKTMKEKQELEDFLKQSAEDSSHQISALVLRAQASEVLLEELQQSFSQAKRDVQEQMAV
LMQSREQVSEELVRLQKDNDSLQGKHSLHVSLQLAEDFILPDTVEVLRELVLKYRENIVHVRTAADHMEE
KLKAEILFLKEQIQAEQCLKENLEETLQLEIENCKEEIVLASISSLKAELERIKVEKGQLESTLREKSQQ
LESLQEMKVNLEEQLKKETAAKATVEQLMFEEKNKAQRLQTELDVSEQVQRDFVKLSQTLQVQLERIRQA
DSLERIRAILNDTKLTDINQLPET

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_019400
ORF Size 2595 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_019400.1, NM_019400.2, NM_019400.3, NP_062273.2
RefSeq Size 5440 bp
RefSeq ORF 2589 bp
Locus ID 54189
UniProt ID O35551
Cytogenetics 11 43.21 cM
MW 99.7 kDa
Gene Summary Rab effector protein acting as linker between gamma-adaptin, RAB4A and RAB5A. Involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. Involved in KCNH1 channels trafficking to and from the cell membrane. Stimulates RABGEF1 mediated nucleotide exchange on RAB5A. Mediates the traffic of PKD1:PKD2 complex from the endoplasmic reticulum through the Golgi to the cilium (PubMed:25405894).[UniProtKB/Swiss-Prot Function]

Other Versions

SKU Description Size
MC222262 Rabep1 (untagged) - Mouse rabaptin, RAB GTPase binding effector protein 1 (Rabep1), (10ug)
    • 10 ug

869,00 EUR

MG217501 Rabep1 (tGFP-tagged) - Mouse rabaptin RAB GTPase binding effector protein 1 (Rabep1), (10ug)
    • 10 ug

992,00 EUR

MR217501L3 Lenti ORF clone of Rabep1 (Myc-DDK-tagged) - Mouse rabaptin, RAB GTPase binding effector protein 1 (Rabep1)
    • 10 ug

1 092,00 EUR

MR217501L4 Lenti ORF clone of Rabep1 (mGFP-tagged) - Mouse rabaptin, RAB GTPase binding effector protein 1 (Rabep1)
    • 10 ug

1 092,00 EUR