Rabbit IgG (H+L), Mouse/Human ads Antibody : Biotin
Cat# OASB01988
Size : 0.5mg
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
Goat Anti-Rabbit IgG(H+L) Antibody - Biotin Conjugated (OASB01988)
| Datasheets/Manuals | Printable datasheet for Goat Anti-Rabbit IgG(H+L) Antibody - Biotin Conjugated (OASB01988) |
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| Tested Species Reactivity | Rabbit |
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| Predicted Species Reactivity | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Host | Goat |
| Conjugation | Biotin |
| Application | FC, IHC-F, IHC-P, ICC, WB |
| Additional Information | Description: Pepsin digest of Goat Anti-Rabbit IgG(H+L), Mouse/Human ads - Pooled antisera from goats hyperimmunized with rabbit IgG; purified by affinity chromatography on pooled rabbit IgG covalently linked to agarose; reacts with the heavy and light chains of rabbit IgG and the light chains of rabbit IgM; cross-adsorbed against mouse and human immunoglobulins and pooled sera for minimal reactivity |
| Reconstitution and Storage | Store at 2-8C |
| Immunogen | Rabbit IgG |
| Purification | Gel filtration of pepsin- digested antibody. |
| Concentration | 0.5 mg/mL |
| Specificity | IgG(H+L) |
| Characterization | To ensure lot- to- lot consistency, each batch of product is tested by ELISA, FLISA, and/or flow cytometry forconformance to characteristics of a standard reference reagent. |
| Warning | Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copiousamounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | Immunofluorescence: ELISA: UNLB, FITC and BIOT conjugates R-PE conjugate AP conjugate HRP conjugate BIOT conjugate <= 1 ug/106 cells <= 0.1 ug/106 cells 1:2,000-1:4,000 1:4,000-1:8,000 1:5,000-1:20,000 |
| Application Info | Direct and indirect immunofluorescent staining, Enzyme-Linked-Immunosorbent-Assay (ELISA), Fluorescent-Linked-Immunosorbent-Assay (FLISA), Immunoblotting, Immunohistochemistry |
| Other Applications Data | Since applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need. |
| Storage | - The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM boratebuffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2- 8 C - The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mMMgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2- 8 C or long- term at - 20 C - The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50%PBS, pH 7.4. No preservative added. Store at 2- 8 C or long- term at - 20 C - The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE) conjugate is supplied as 0.25 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze! - Protect fluorochrome- conjugated forms from light. Reagents are stable for the period shown on the label ifstored as directed. |
| Cross Absorption | Pooled human sera, purified human paraproteins, pooled mouse sera, andpooled mouse plasmacytoma/hybridoma proteins. |
| Gene Full Name | Ig gamma-1 chain C region |
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