Rabbit IgG (H&L) Antibody - HRP Conjugated
Cat# OARA04989
Size : 2mg
Brand : Aviva Systems Biology
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Goat Anti-Rabbit IgG (H&L) Polyclonal Antibody - HRP Conjugated (OARA04989)
| Datasheets/Manuals | Printable datasheet for Goat Anti-Rabbit IgG (H&L) Polyclonal Antibody - HRP Conjugated (OARA04989) |
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| Predicted Species Reactivity | Rabbit |
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| Product Format | Lyophilized. 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugation | HRP |
| Application | ELISA|IHC|WB |
| Additional Information | Conjugation Chemistry: Periodate |
| Reconstitution and Storage | -20°C|2°C to 8°C |
| Immunogen | Rabbit IgG whole molecule |
| Purification | RABBIT IgG (H&L) Secondary Antibody Peroxidase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads. |
| Concentration | 2 mg/ml |
| Specificity | Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG and Rabbit Serum. |
| Application Info | ELISA 1:150000~250000 IHC 1:500~2500 WB 1:10000~20000 This anti-rabbit HRP secondary antibody is suitable for ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation, Immunohistochemistry as well as other HRP antibody based assays. Specific conditions for reactivity and signal detection should be optimized by the end user. |
| Description of Target | Anti-Rabbit IgG Antibody peroxidase generated in goat detects rabbit IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Rabbit IgG (H&L) is conjugated to peroxidase. |
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| Molecular Weight | 40 kDa |