QuantiFluo™ α-Amylase Inhibitor Screening Kit
Application
- For evaluation of drugs and screening potential inhibitors of α-amylase.
Key Features
- Safe. Non-radioactive assay.
- Fast and convenient. Homogeneous “mix-incubate-measure” type assay. Can be completed in under an hour at room temperature.
- Robust and High-throughput. A Z’-factor of >0.90 was observed in a 384-well format. Can be readily automated to assay thousands of samples per day.
Method
- FP485/520 nm
Samples
- Compounds that affect α-amylase activity
Species
- All
Procedures
- 40 min
Size
- 400 tests in a 384-well plate
Detection Limit
- NA
Shelf Life
- 6 months
More Details
AMYLASE belongs to the family of glycoside hydrolase enzymes that break down starch into glucose molecules by acting on α-1,4-glycosidic bonds. The α-amylases (EC 3.2.1.1) cleave at random locations on the starch chain, ultimately yielding maltotriose and maltose, glucose, and “limit dextrin” from amylose and amylopectin. In mammals, α-amylase is a major digestive enzyme. Increased enzyme levels in humans are associated with salivary trauma, mumps due to inflammation of the salivary glands, pancreatitis, and renal failure.Simple, direct, and automation-ready procedures for measuring α-amylase inhibition are highly desirable in Research and Drug Discovery. BioAssay Systems’ QuantiFluo™ α-Amylase Inhibitor Screening Kit utilizes fluorescence polarization (FP) to screen for potential α-amylase inhibitors. In this assay, α-amylase cleaves a fluorescent amylose substrate. The decrease in FP is directly proportional to the α-amylase activity in the sample. Inhibition is therefore determined by the increase in FP (λex/em = 485/520 nm).Does this kit come with α-amylase?
No, this kit only comes with the fluoresence polarization reagent.
What equipment should I use?
A fluorescence polarization-capable plate reader that can read 485/520 nm.
What buffer should I use with this kit?
While our reagent was developed using Porcine α-amylase and 20 mM KPi, 50 mM NaCl, pH 7.0 buffer, you should use the buffer that is optimal for your enzyme.
For more detailed product information and questions, please feel free to email or call us at 1-510-782-9988 x 2. For more general information regarding our assays, please refer to Technical Support.
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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.
– Fast turnaround
– Quality data
– Low cost