ProteinFind® Anti-Cytochrome C Mouse Monoclonal Antibody

Cat# HA103-01

Size : 20ul

Brand : TransGen Biotech

Contact local distributor :


Phone : +1 850 650 7790

  • ProteinFind® Anti-Cytochrome C Mouse Monoclonal Antibody.jpg
Product Details

Cytochrome C is an electron transfer protein located in the mitochondrial membrane space and is an essential component of the cell's electron transfer chain. When the cell is stimulated by the apoptosis signal, Cytochrome C is released from the mitochondria to the cytoplasm, accompanied by a series of changes in membrane potential, osmotic pressure and ultrastructure, promoting the activation of apoptotic caspases required for cell apoptosis.


Species reactivity 

Human, mouse, rats, guinea pigs, hamsters and chlorocebus sabaeus(Species reactivity is based on WB experiment)


Isotype: Mouse IgG2a 


Immunogen

• Recombinant human Cytochrome C full-length protein

• Entrez Gene ID: 54205

• UniProt ID: P99999


Applications and Suggested Dilution

• Western Blot: 1:1000-5000 dilution.

• IP: 1:100 dilution.

• IF: 1:100-200 dilution.

• FC: 1:100 dilution.

• IHC: 1:100-500 dilution(use Tris-EDTA for retrieval).


Storage

PBS (pH 7.4), 0.02% Sodium Azide, 50% Glycerol; at -20℃ for two years, avoid repeated freezing and thawing.


Shipping

Dry ice (-70 ℃)

Citations

[1]. Li K, Li Y, Shelton JM, Richardson JA, Spencer E, Chen ZJ,  et al. Cytochrome c Deficiency Causes Embryonic Lethalityand Attenuates Stress-Induced Apoptosis[J]. Cell. 2000, 101 (4): 389-99.

[2]. Heiskanen KM, Bhat MB, Wang HW, Ma,  Nieminen AL. Mitochondrial depolarization accompanies cytochrome c release during apoptosis in PC6 cells[J]. Journal of Biological Chemistry. 1999, 274 (9): 5654-8

[3]. Liu X, Kim CN, Yang J, Jemmerson R, Wang X. Induction of apoptotic program in cell-free extracts: requirement for dATP and cytochrome c[J]. Cell. 1996, 86 (1): 147-57.