POLR2B Antibody - middle region : Biotin
Cat# ARP32456_P050-Biotin
Size : 100ul
Brand : Aviva Systems Biology
Contact local distributor :
Phone : +1 850 650 7790
POLR2B Antibody - middle region : Biotin (ARP32456_P050-Biotin)
| Datasheets/Manuals | Printable datasheet for anti-POLR2B (ARP32456_P050-Biotin) antibody |
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| Publications | Lee, J.-H. & Skalnik, D. G. Wdr82 is a C-terminal domain-binding protein that recruits the Setd1A Histone H3-Lys4 methyltransferase complex to transcription start sites of transcribed human genes. Mol. Cell. Biol. 28, 609-18 (2008). WB, Bovine, Human, Dog, Horse, Rabbit, Rat, Guinea pig, Mouse, Zebrafish 17998332 |
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| Predicted Species Reactivity | Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish |
| Product Format | Liquid. Purified antibody supplied in 1x PBS buffer. |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugation | Biotin |
| Application | WB |
| Reconstitution and Storage | All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding. |
| Immunogen | The immunogen is a synthetic peptide directed towards the middle region of human POLR2B |
| Purification | Affinity Purified |
| Predicted Homology Based on Immunogen Sequence | Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 93% |
| Peptide Sequence | Synthetic peptide located within the following region: FFRSVFYRSYKEQESKKGFDQEEVFEKPTRETCQGMRHAIYDKLDDDGLI |
| Concentration | 0.5 mg/ml |
| Blocking Peptide | For anti-POLR2B (ARP32456_P050-Biotin) antibody is Catalog # AAP32456 (Previous Catalog # AAPP03452) |
| Subunit | RPB2 |
| Reference | Zhou,M., et al., (2003) Proc. Natl. Acad. Sci. U.S.A. 100 (22), 12666-12671 |
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| Gene Symbol | POLR2B |
| Gene Full Name | Polymerase (RNA) II (DNA directed) polypeptide B, 140kDa |
| Alias Symbols | RPB2, POL2RB, hRPB140 |
| NCBI Gene Id | 5431 |
| Protein Name | DNA-directed RNA polymerase II subunit RPB2 |
| Description of Target | POLR2B encodes the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and the newly synthesized RNA. |
| Uniprot ID | P30876 |
| Protein Accession # | NP_000929 |
| Nucleotide Accession # | NM_000938 |
| Protein Size (# AA) | 1174 |
| Molecular Weight | 134kDa |
| Protein Interactions | ESR1; UBC; TCEB3; SRC; PSMB9; SUMO2; XRN2; WWOX; SUZ12; RNF2; NEDD4; ITCH; RABEP2; HECW2; FBXO6; CDK20; HDAC11; ILK; POLR2C; MED19; MED26; RECQL5; VCP; BARD1; MDC1; POLR3F; TUBB4B; TUBA4A; POLR2I; POLR2E; POLR2D; POLR2A; DCTN1; ATP6V1B2; MEN1; CDK7; CCNH; |
