PMA Real-Time PCR Bacterial Viability Kit - Salmonella enterica (invA)
Cat# 31033
Size : 1kit
Brand : Biotium
Contact local distributor :
Phone : +1 850 650 7790
PMA Real-Time PCR Bacterial Viability Kit – Salmonella enterica (invA)
A kit designed to perform viability PCR on Salmonella enterica. It contains a viability PCR dye (PMAxx™ or PMA) and primers to amplify the invA gene.
Product Attributes
| Viability dye | PMA, PMAxx™ |
|---|---|
| Storage Conditions | Store at -10 to -35 °C |
| Assay type/options | Live/dead discrimination, Viability PCR |
| Detection method/readout | PCR/qPCR |
Technology
Microbiology Products
Viability PCR
The Full Spectrum Blog
Blog: 12 years later: Viability PCR using PMA and PMAxx™ dyes
Blog: Switching from agar plates to v-PCR for bacteria viability testing – What you need to know
Product Description
This kit is designed to perform viability PCR on Salmonella enterica. It contains a viability PCR dye (PMA or PMAxx™) and primers to amplify the invA gene.
Features
- Selectively detect viable Salmonella enterica using qPCR
- Contains primers to amplify the invA gene
- Works even in complex samples or mixed cultures
- Sufficient reagents to treat 80 bacterial cultures with PMA or PMAxx™
- Sufficient reagents for 200 PCR reactions
Kit Components
- PMA dye or PMAxx™ dye
- PMA Enhancer for Gram Negative Bacteria
- Forget-Me-Not™ EvaGreen® qPCR Master Mix
- ROX reference dye
- invA primer mix, 5 uM
About this kit
Salmonella enterica is a gram-negative bacteria that causes the food-borne illness salmonellosis. PMA-based viability PCR for S. enterica has been reported using the primers provided in the kit. In addition, these primers have been validated at Biotium for real-time qPCR using Forget-Me-Not™ EvaGreen® qPCR Master Mix.
To learn more about the advantages of determining bacterial viability using viability PCR, visit the Viability PCR Technology Page.
Strain-Specific Bacterial Viability PCR Kits
| Bacteria Strain | Gene name | Kit with PMA (catalog #) | Kit with PMAxx™ (catalog #) |
|---|---|---|---|
| Salmonella enterica | invA | 31033 | 31033-X |
| Mycobacterium tuberculosis | groEL2 | 31034 | N/A |
| Staphylococcus aureus | nuc | 31035 | N/A |
| MRSA | mecA | 31036 | N/A |
| E. coli O157:H7 | Z3276 | 31037 | 31037-X |
| E. coli | uidA | 31050 | 31050-X |
| Listeria monocytogenes | hly | 31051 | 31051-X |
| Legionella pneumophila | mip | 31053 | N/A |
Don’t see your favorite strain? Let us know at info@biotrend.com.
PMAxx technology is covered by granted and/or pending US and international patents.
References
Download PMA and PMAxx™ References and a list of PMA and PMAxx™ Validated Bacterial Strains.