Pigeon IgG (H+L chain) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, IHC, WB
Recommended Dilution	Can be used in enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG, antigen or antibody, of appropriately treated cell and tissue substrates at the cellular and subcellular level. In nonisotopic assay methodology (e.g. ELISA) to identify and measure a specific IgG in pigeon serum or other body fluid. In electron microscopy, since the complex between the conjugated antibody and the antigen also has electron-dense properties. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemical and cytochemical Use: 1/100-1/500. ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/10000.
Reactivities	Pigeon
Host	Rabbit
Isotype	IgG
Clonality	Polyclonal
Immunogen	Purified normal IgG isolated from pooled pigeon serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity	The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in pigeon serum, as tested by immunoelectrophoresis and double radial immunodiffusion. Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. The degree of cross-reactivity depends in addition to the phylogenetic relationship also on the concentration of the reactants and the sensitivity of the assay format. Cross-reactivity of this antiserum has not been tested in detail, however in immunoelectrophoresis a reaction with chicken, duck and turkey has been observed.
Formulation	PBS, pH 7.2, No preservative added, as it may interfere with the antibody activity. No foreign proteins added. Label: HRP State: Lyophilized purified hyperimmune Ig fraction Label: Horseradish Peroxidase Molar radio: 1.7
Reconstitution Method	Restore with 1 ml sterile distilled water
Concentration	lot specific
Purification	DEAE-column Chromatography
Conjugation	HRP
Storage	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Synonyms	Pigeon Immunoglobulin G
Reference Data