Specifications

Product Data
N-Tag	GST
E. coli Selection	Ampicillin (100 ug/mL)
Features	Upon IPTG induction, ORFs cloned in this vector can be expressed in E.coli (BL21/DE3) as a tagged protein with an N-terminal GST tag. Detection and purification of the transgene are best done using a glutathione affinity column. If the N-terminal tagging interferes with the protein's function, you can choose the C-terminal GST-tagging vector, PS100083 PS100028_F Primer can be used to sequence the target sequence after cloning. PS100028_R Primer can be used to sequence the target sequence after cloning.
Schematic of the multiple cloning sites