Application
| FC |
|---|---|
| Isotype | Mouse IgG2a, kappa |
| Concentration | 0.2 mg/mL |
| Reactivity | Mouse |
| Formulation | 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2 |
| Host | Mouse |
| Gene ID | 17059 |
|---|---|
| Gene Name | Klrb1c |
| Alternative Name(s) | CD161, NKR-P1C, Ly-55 |
| Format | PerCP-Cy5.5 |
| Preparation | This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. It is recommended to store the product undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze. |
| Application Notes | This antibody preparation has been quality-tested for flow cytometry using mouse spleen cells, or an appropriate cell type (where indicated). The amount of antibody required for optimal staining of a cell sample should be determined empirically in your system. |
| Storage Conditions | 2-8°C protected from light |
Krebs DL, Chehal MK, Sio A, Huntington ND, Da ML, Ziltener P, Inglese M, Kountouri N, Priatel JJ, Jones J, Tarlinton DM, Anderson GP, Hibbs ML, and Harder KW. 2012. J. Immunol. 188:5094-5105. (in vivo depletion)
Lubinski JM, Lazear HM, Awasthi S, Wang F, and Friedman HM. 2011. J. Virol. 85(7): 3239-3249. (in vivo depletion)
Diamond MS, Kinder M, Matsushita H, Mashayekhi M, Dunn GP, Archambault JM, Lee H, Arthur CD, White JM, Kalinke U, Murphy KM, and Schreiber RD. 2011. J. Exp. Med. 208: 1989-2003. (in vivo depletion)
Awasthi A, Samarakoon A, Chu H, Kamalakannan R, Quilliam LA, Chrzanowska-Wodnicka M, White GC, and Malarkannan S. 2010. J. Exp. Med. 207: 1923-1938. (in vitro activation)
Coudert JD, Scarpellino L, Gros F, Vivier E, and Held W. 2008 Blood. 111: 3571-3578. (Immunoprecipitation)
Ljutic B, Carlyle JR, Filipp D, Nakagawa R, Julius M, and Zuniga-Pflucker JC. 2005. J. Immunol. 174: 4789-4796. (Immunoprecipitation)
Kanwar JR, Shen W-P, Kanwar RK, Berg RW, and Krissansen GW. 2001. J. Natl. Cancer Inst. 93: 1541-1552. (Immunohistochemistry – frozen tissue, Immunofluorescence microscopy – frozen tissue, in vivo depletion)