Application
| FC |
|---|---|
| Isotype | Rat IgG2b |
| Concentration | 0.2 mg/mL |
| Reactivity | Mouse |
| Formulation | 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2 |
| Host | Rat |
| Gene ID | 12525 |
|---|---|
| Gene Name | Cd8a |
| Alternative Name(s) | CD8 alpha, Ly-2, Ly-35, Ly-B, Lyt-2 |
| Format | PerCP-Cy5.5 |
| Preparation | This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. It is recommended to store the product undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze. |
| Application Notes | This antibody preparation has been quality-tested for flow cytometry using mouse spleen cells, or an appropriate cell type (where indicated). The amount of antibody required for optimal staining of a cell sample should be determined empirically in your system. |
| Storage Conditions | 2-8°C protected from light |
Lin J-S, Szaba FM, Kummer LW, Chromy BA, and Smiley ST. 2011. J. Immunol. 187: 897-904. (in vivo depletion)
Wozniak KL, Young ML, and Wormley FL. 2011. Clin. Vaccine Immunol. 18(5):717-723. (in vivo depletion)
Hufford MM, Kim TS, Sun J, and Braciale TJ. 2011. J. Exp. Med. 208: 167-180 (in vivo depletion)
Ou R, zhang M, Huang L, Flavell RA, Koni PA, and Moskophidis D. 2008. J. Virol. 82:2952-2965. (Immunohistochemistry – OCT embedded frozen tissue)
Bosselut R, Zhang W, Ashe JM, Kopacz JL, Samelson LE, and Singer A. 1999. J. Exp. Med. 190: 1517-1526. (in vitro activation)
Davies A, Kalb, S, Liang B, Aldrich CJ, Lemonnier FA, Jiang H, Cotter R, and Soloski MJ. 2003. J. Immunol. 170: 5027-5033. (Blocking)