pEASY®-T3 Cloning Kit
Cat# CT301-01
Size : 20rxns
Brand : TransGen Biotech
Contact local distributor :
Phone : +1 850 650 7790
ManualpEASY®- T3 Cloning Vector provides dual EcoR I and dual Not I enzyme sites. It is designed for cloning and sequencing Taq-amplified PCR products. The cloned insert can be released from a single enzyme digestion.
•5 minutes fast ligation of Taq-amplified PCR products.
•Ampicillin resistance genes for selection.
•Easy blue/white selection.
•T7 promoter, SP6 promoter, M13 forward and M13 reverse primers for sequencing.
•T7 promoter and SP6 promoter for in vitro transcription.
•Trans1-T1 Phage Pesistant Chemically Competent Cells, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing.
Storage
Competent Cell at -70°C for six months; others at -20°C for nine months
Shipping
Dry ice (-70℃)
| Component | CT301-01 (20 rxns) | CT301-02 (60 rxns) |
| pEASY ®--T3 Cloning Vector (10 ng/μl) | 20 μl | 3×20 μl |
| Control Template (5 ng/μl) | 5 μl | 5 μl |
| Control Primers (10 μM) | 5 μl | 5 μl |
| M13 Forward Primer (10 μM) | 50 μl | 150 μl |
| M13 Reverse Primer (10 μM) | 50 μl | 150 μl |
| Trans1-T1 Phage Resistant Chemically Competent Cells | 10×100 μl | 30×100 μl |
Overexpression of microRNA OsmiR397 improves rice yield by increasing grain size and promoting panicle branching[J]. Nature Biotechnology, 2013, 31(9):848-852.
Wang J, Wang L, Feng G, et al. Asymmetric Expression of LincGET Biases Cell Fate in Two-Cell Mouse Embryos[J]. Cell, 2018, 13, 175(7):1887-1901.
Zhang YC, Liao JY, Li ZY, et al. Genome-wide screening and functional analysis identify a large number of long noncoding RNAs involved in the sexual reproduction of rice[J]. Genome Biology, 2014, 15(12):512.
Chen Z H , Wang W T , Huang W , et al. The lncRNA HOTAIRM1 regulates the degradation of PML-RARA oncoprotein and myeloid cell differentiation by enhancing the autophagy pathway[J]. Cell Death and Differentiation, 2017, 24(2):212-224.
Peng S, Feng Y, Wang Y, et al. Prevalence of antibiotic resistance genes in soils after continually applied with different animal manure for 30 years[J]. J Hazard Mater. 2017, 15(340):16-25.