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> p23 Antibody

p23 Antibody

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Cat# ASM10083-A655-100

Size : Onrequest

Brand : Abcepta

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Phone : +1 850 650 7790

 
  • ICC/IF - p23 Antibody ASM10083
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-p23 Monoclonal Antibody, Clone JJ6 (ASM10083). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-p23 Monoclonal Antibody (ASM10083) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-p23 Antibody. (C) Composite.
  • IHC - p23 Antibody ASM10083
    Immunohistochemistry analysis using Mouse Anti-p23 Monoclonal Antibody, Clone JJ6 (ASM10083). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-p23 Monoclonal Antibody (ASM10083) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermal, dermal, HF, muscle staining. Bright dermal staining.
  • WB - p23 Antibody ASM10083
    Western Blot analysis of Human Cell lysates showing detection of p23 protein using Mouse Anti-p23 Monoclonal Antibody, Clone JJ6 (ASM10083). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-p23 Monoclonal Antibody (ASM10083) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
  • ICC/IF - p23 Antibody ASM10083
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-p23 Monoclonal Antibody, Clone JJ6 (ASM10083). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-p23 Monoclonal Antibody (ASM10083) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Uniform epidermal staining – some evidence of cell-cell borders.
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, ICC, IP, E, AM
Primary Accession Q15185
Other Accession NP_006592.3
Host Mouse
Isotype IgG1
Reactivity Human, Mouse, Rabbit, Chicken, Yeast, Guinea Pig
Clonality Monoclonal
Description Mouse Anti-Human p23 Monoclonal IgG1
Target/Specificity Detects ~23kDa.
Other Names co chaperone p23 Antibody, PTGES3 Antibody, TEBP Antibody, telomerase binding protein p23 Antibody, unactive progesterone receptor 23kDa Antibody, HSP90 co-chaperone Antibody, Progesterone receptor complex p23 Antibody, Cytosolic prostaglandin E2 synthase Antibody
Clone Names JJ6
Immunogen Recombinant human full length p23 protein
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS, 50% glycerol, 0.09% sodium azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis 0.5 µg/ml of SMC-156 was sufficient for detection of p23 in 20 µg of heat shocked cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Cellular Localization Cytoplasm