K2099-200 assays | Nitric Oxide Fluorometric Assay Kit Biotrend

Background

Nitric oxide is essential in neurotransmission, vascular regulation, immune response and apoptosis. Because Nitric oxide is rapidly converted into nitrite and nitrate, the NO production can be quantitatively determined by the total concentration of nitrite and nitrate. This kit is a simple and accurate two-step measurement of total nitrate/nitrite concentration. Nitrate reductase converts nitrate into nitrite first, and then nitrite reacts with the fluorescent probe DAN (2, 3 diaminonaphthalene). NaOH enhances the fluorescent yield. The total nitric oxide production is proportional to the fluorescent intensity. The kit has been tested with culture media, plasma, and tissue homogenates.

Features & Properties

Features	Simple procedure; Takes only 2-3 hours; Fast and convenient.
Shipping	Gel pack.
Storage Conditions	Store at -20°C.

Quality Control

DataSheet

Storage

Store at -20°C.

Overview

Kit components

Assay Buffer, Enzyme Cofactor, Enhancer, Nitrate Reductase, Nitrate Standard, Nitrite Standard, DAN Probe, Sodium Hydroxide, Microtiter plate, Plate Cover

Nitric Oxide Fluorometric Assay Kit

Cat# K2099-200 assays

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