IMAC SuperSpin is supplied as a low cost disposable spin-column that allows rapid purification and screening of histidine-tagged proteins at a low cost. The spin tube is filled with novel metal immobilised chromatography resin of small particles (20 – 50 µm). It gives fast mass transfer rate of target proteins, so decent amounts of protein can be captured in a short solid / liquid contact time. Three types of spin tubes are available; Ni SuperSpin, Co SuperSpin and Zn SuperSpin, for fast screening of the best immobilised metal ion to a given target protein. It is a particularly powerful tool in applications such as small-scale purification, high-throughput screening, purification process optimisation etc.

Key benefits:

• Fast purification of many protein samples with a standard microcentrifuge
• High protein binding capacity
• Very low cost in comparison to similar products in the marketplace
• Optimisation of the binding, washing and elution conditions (e.g. imidazole concentration, pH, salt concentration, detergents etc) in one or two sets of experiments in short periods and at very low cost
• Direct processing of clarified or unclarified protein sample
• Full operational manual supplied

The fine IMAC particles are very stable and compatible with a variety of chemical reagents (e.g. denaturing reagents and reducing reagents etc).