Neutrophil Elastase (ELANE) (NM_001972) Human Untagged Clone
CAT#: SC118911
ELANE (untagged)-Human elastase, neutrophil expressed (ELANE)
Product Images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Symbol | Neutrophil Elastase |
| Synonyms | ELA2; GE; HLE; HNE; NE; PMN-E; SCN1 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | >OriGene ORF within SC118911 sequence for NM_001972 edited (data generated by NextGen Sequencing) ATGACCCTCGGCCGCCGACTCGCGTGTCTTTTCCTCGCCTGTGTCCTGCCGGCCTTGCTG CTGGGGGGCACCGCGCTGGCCTCGGAGATTGTGGGGGGCCGGCGAGCGCGGCCCCACGCG TGGCCCTTCATGGTGTCCCTGCAGCTGCGCGGAGGCCACTTCTGCGGCGCCACCCTGATT GCGCCCAACTTCGTCATGTCGGCCGCGCACTGCGTGGCGAATGTAAACGTCCGCGCGGTG CGGGTGGTCCTGGGAGCCCATAACCTCTCGCGGCGGGAGCCCACCCGGCAGGTGTTCGCC GTGCAGCGCATCTTCGAAAACGGCTACGACCCCGTAAACTTGCTCAACGACATCGTGATT CTCCAGCTCAACGGGTCGGCCACCATCAACGCCAACGTGCAGGTGGCCCAGCTGCCGGCT CAGGGACGCCGCCTGGGCAACGGGGTGCAGTGCCTGGCCATGGGCTGGGGCCTTCTGGGC AGGAACCGTGGGATCGCCAGCGTCCTGCAGGAGCTCAACGTGACGGTGGTGACGTCCCTC TGCCGTCGCAGCAACGTCTGCACTCTCGTGAGGGGCCGGCAGGCCGGCGTCTGTTTCGGG GACTCCGGCAGCCCCTTGGTCTGCAACGGGCTAATCCACGGAATTGCCTCCTTCGTCCGG GGAGGCTGCGCCTCAGGGCTCTACCCCGATGCCTTTGCCCCGGTGGCACAGTTTGTAAAC TGGATCGACTCTATCATCCAACGCTCCGAGGACAACCCCTGTCCCCACCCCCGGGACCCG GACCCGGCCAGCAGGACCCACTGA Clone variation with respect to NM_001972.2 >OriGene 5' read for NM_001972 unedited GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGCCGGGCGGGCACGGAGG GGCAGAGACCCCGGAGCCCCAGCCCCACCATGACCCTCGGCCGCCGACTCGCGTGTCTTT TCCTCGCCTGTGTCCTGCCGGCCTTGCTGCTGGGGGGCACCGCGCTGGCCTCGGAGATTG TGGGGGGCCGGCGAGCGCGGCCCCACGCGTGGCCCTTCATGGTGTCCCTGCAGCTGCGCG GAGGCCACTTCTGCGGCGCCACCCTGATTGCGCCCAACTTCGTCATGTCGGCCGCGCACT GCGTGGCGAATGTAAACGTCCGCGCGGTGCGGGTGGTCCTGGGAGCCCATAACCTCTCGC GGCGGGAGCCCACCCGGCAGGTGTTCGCCGTGCAGCGCATCTTCGAAAACGGCTACGACC CCGTAAACTTGCTCAACGACATCGTGATTCTCCAGCTCAACGGGTCGGCCACCATCAACG CCAACGTGCAGGTGGCCCAGCTGCCGGCTCAGGGACGCCGCCTGGGCAACGGNGTGCAGT GCCTGGCCATGGGCTGNNGCCTTTCTGGCAGGAACCGTGGGATCNGCAGCGTCCTGCAGG AGCTCAACGTGACGGTGGTGACGTCCCTCTGCCGTCGCAGCAACGTCTGCACTCTCGTGA GGGGCCCNGCAGCCCGCCGTCTGNTTCGGGGACTCCNGCAGCCCCTGGGTCTGNCACGGG CTAATCCACGGAANTGGCTCCTTCGTCCGGGGGAGGCTGCGCCTCCAGGCCTCTACCCCG ATGCCCTTGCCCCCGTGGNCACACGTTGTAAACTGGATCGACTTCTATATCCAACGCTCC GGAGACAACCCCTGTCCCCACCCCCGGAACCCGGACCCGGCCAAAGGACCACTTGAAAGG TCTGCCCGGGTACCTAAGTTGGCCCAACCCACCTCTCAAGATTGGACCATAACATTTTGG TTTGTAAAAAAAAAAAAAAAAAAACATCATCCTTAATGGGGCCGGGCTTAATTGTTCCTG ACATACCCGGGGGGACCTTGGACTCTCCAGGGCTCCGTGG >OriGene 3' read for NM_001972 unedited GGCCCGGGGGGNNNNCNNNNNNNTTTANNNNNNNCGGTNTTACTCTGANCCGCGGNCCGC ATACNAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTACAAAACTAAAAAGTTTATTGT GCCAGATGCTGGAGAGTGTGGGTGTGGGCAGCTGAGGTGACCCGGGCAGCCCTTCTCAGT GGGTCCTGCTGGCCGGGTCCGGGTCCCGGGGGTGGGGACAGGGGTTGTCCTCGGAGCGTT GGATGATAGAGTCGATCCAGTTTACAAACTGTGCCACCGGGGCAAAGGCATCGGGGTAGA GCCCTGAGGCGCAGCCTCCCCGGACGAAGGAGGCAATTCCGTGGATTAGCCCGTTGCAGA CCAAGGGGCTGCCGGAGTCCCCGAAACAGACGCCGGCCTGCCGGCCCCTCACGAGAGTGC AGACGTTGCTTGCGACGGCAGAGGGACGTTACCACCGTCACGTTGAGCTCCTGCAGGAAC CTGGCGAACCCACGGTTCCTGCCCAAAAGCCCCCACCCATGGGCCCGGCCCTTGCCCCCC TTGCCCAAGGCGGGGTCCCTTAACCGTACATTTGGGCCCCCCTGCACCTTTGGGGTTTGA AGGGGGCCCCACCCCTTCTCATTTCTGAAAAAAAAATAATTTTTTTAAAAAATTTCGGGG GGGCTAACCCTTTTTAAAAAATCCCTTTGTCGGGAAAAAACCCCCCGCGGTGGGCCCCCC CCCCCAGAAGGGTTTGGTGGTTAAAAAAAACACCCCCCCCTCGTGGGGGGGTTTTTATTT CTTTCCCCCCGAGGGGCGGGCGGCCAAAAAAAAAAAAATAGGGCACCCCCAACGGGGGGG GTGGCCCCAAATATAACGTTTTCCCCTCCCTCTTTTGTGGCGGCACCACAAAATATAGCA CCCTCCCCGTGGGGGCGCTTCTCCCTTCTCCCCCCTC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_001972 |
| Insert Size | 804 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001972.2, NP_001963.1 |
| RefSeq Size | 938 bp |
| RefSeq ORF | 804 bp |
| Locus ID | 1991 |
| UniProt ID | P08246 |
| Cytogenetics | 19p13.3 |
| Domains | Tryp_SPc |
| Protein Families | Protease, Transmembrane |
| Protein Pathways | Systemic lupus erythematosus |
| Gene Summary | Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode structurally similar proteins. The encoded preproprotein is proteolytically processed to generate the active protease. Following activation, this protease hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix. The enzyme may play a role in degenerative and inflammatory diseases through proteolysis of collagen-IV and elastin. This protein also degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is present in a gene cluster on chromosome 19. [provided by RefSeq, Jan 2016] |
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Resources
Other Versions
| SKU | Description | Size | |
|---|---|---|---|
| RC210399 | ELANE (Myc-DDK-tagged)-Human elastase, neutrophil expressed (ELANE) | 300,00 EUR | |
| RC210399L1 | Lenti ORF clone of Human elastase, neutrophil expressed (ELANE), Myc-DDK-tagged | 600,00 EUR | |
| RC210399L2 | Lenti ORF clone of Human elastase, neutrophil expressed (ELANE), mGFP tagged | 600,00 EUR | |
| RC210399L3 | Lenti ORF clone of Human elastase, neutrophil expressed (ELANE), Myc-DDK-tagged | 600,00 EUR | |
| RC210399L4 | Lenti ORF clone of Human elastase, neutrophil expressed (ELANE), mGFP tagged | 600,00 EUR | |
| RG210399 | ELANE (tGFP-tagged) - Human elastase, neutrophil expressed (ELANE) | 500,00 EUR |