Mycobacterium tuberculosis (all antigens) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IF, IHC, WB
Recommended Dilution	Suitable for use with avidin and streptavidin amplification systems for Immunohistochemistry and IFA. Also suitable for Western blot and ELISA.
Reactivities	Mycobacterium tuberculosis
Host	Rabbit
Clonality	Polyclonal
Immunogen	Purified PPD.
Specificity	Minimum of two major M. tuberculosis bands by immunoelectrophoresis (gamma & beta). This antibody has not been absorbed and may react with related microorganisms. Reactive with other Mycobacteria species including M. avium, M. phlei and M. parafortuitum. Antibody is non-reactive with E. coli K12, Salmonella typhimurium, Pseudomonas aeruginosa, Streptococcus (group B), Candida albicans and Neisseria meningitidis.
Formulation	0.01 M PBS, pH 7.2 containing 0.09% Sodium Azide as preservative without stabilizing proteins. Label: Biotin State: Liquid purified Ig fraction. Label: Covalently coupled with the N-Hydroxysuccinimide ester of under mild conditions to give a high degree of substitution
Concentration	lot specific
Conjugation	Biotin
Storage	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
Stability	Shelf life: one year from despatch.
Background	Mycobacterium tuberculosis is the most common cause of tuberculosis. Primary infection begins with inhalation of 1 to 10 aerosolised bacilli. The pathogenicity of the organism is determined by its ability to escape host immune responses as well as eliciting delayed hypersensitivity. Alveolar macrophages engulf the invading cells but are unable to mount an effective defense. Several virulence factors are responsible for this apparent failure; most notably in the mycobacterial cell wall are the cord factor, lipoarabinomannan, and the 65 kd heat shock protein or HSP65. The emergence of new strains of resistant Mycobacterium tuberculosis has created new interest in clinical diagnosis. Studies have shown immunohistochemical techniques to be superior to conventional special stains. Thus the demonstration of mycobacterial antigens are not only useful in establishing mycobacterial aetiology, but can also be used as an alternative method to the conventional Ziehl-Neelsen method.
Synonyms	M. tuberculosis, TB
Reference Data